Application of bitter gourd exosome in radiation heart injury protection

Abstract

The invention discloses application of bitter gourd exosomes in radiation heart injury protection, the bitter gourd exosomes and rat myocardial cells (H9C2) are co-cultured and subjected to radiation treatment, MTT experiments show that 5-25 [mu] g/ml of the bitter gourd exosomes can promote the cell viability of the radiated H9C2 myocardial cells, and 10 [mu] g/ml of the bitter gourd exosomes are selected as the use concentration of the bitter gourd exosomes. Under the condition that the bitter gourd exosome is 10 [mu] g/ml, Ki-67 fluorescent staining proves that the bitter gourd exosome can promote proliferation of radiated H9C2 myocardial cells. An Annexin V-PE/7-AAD flow apoptosis experiment and an apoptosis protein cleaved-caspase3 immunoblotting test prove that the bitter gourd exosome can be used for reducing the apoptosis of H9C2 myocardial cells after radiation. Immunofluorescence staining and protein immunoblotting of a DNA damage marker gamma-H2A. X show that the bitter gourd exosome can reduce DNA damage of H9C2 myocardial cells after radiation.

Description

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AI Technical Summary

Benefits of technology

This extract helps improve cell growth during radiotherapy treatment for cancer patients with high risk factors such as breast or lung tumors that are associated with increased chances of developing complications later on when they develop new problems like brain tissue damaging them due to ionizing ray exposure.

Problems solved by technology

The technical problem addressed in this patents relates to developing new types of radioprotectors or agents containing specific components without harming beneficial healthy tissues like bone marrow during medical treatments while also having improved effectiveness against diseases caused by ionizing ray exposure (ray) compared to traditional methods involving drugs or other substances used alone.

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