Nano material capable of trapping erythrocytes

A monocyte and cell technology, which is applied in the field of capturing rare cells in blood based on biocompatible nanoparticle substrates, can solve the problems of high requirements for instruments and equipment, high technical difficulty, and difficulty in promotion, and achieves efficient capture, low price, enhanced Capture the effect of the effect

Inactive Publication Date: 2015-11-11
石莹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology has high separation purity, but it is difficult to popularize because of its high technical difficulty and high requirements for equipment.

Method used

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  • Nano material capable of trapping erythrocytes
  • Nano material capable of trapping erythrocytes
  • Nano material capable of trapping erythrocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] according to figure 1 One embodiment of the present invention shown is a method for capturing fetal nucleated red blood cells using a biocompatible TiO2 nanoparticle substrate. First, enter the preparation operation of TiO2 nanoparticles S1100: first use hydrolysis hydrothermal to prepare a TiO2 nanoparticle precursor solution, and use titanium Hydrolyze tetrabutyl ester in deionized water, then use the hydrothermal method to synthesize nanoparticles 200 with a particle size of 200-500nm, and disperse 1g of synthesized TiO2 particles into 0.05g of lauric acid, 0.2g of ethyl cellulose and 10ml After mixing the mixed solution of terpineol and ethanol (volume ratio 1:1), dilute it in absolute ethanol to make a TiSP precursor solution with a concentration of 5 mg / ml, and then take a piece of clean glass 100 and mix it in concentrated H2SO4 and H2O2 (Volume ratio is 3:1) Soak in the mixed solution for 1.5 hours, then rinse with a large amount of deionized water and dry on a ...

Embodiment 2

[0042] according to figure 1 One embodiment of the present invention shown is a method for capturing fetal nucleated red blood cells using a biocompatible TiO2 nanoparticle substrate. First, enter the preparation operation of TiO2 nanoparticles S1100: first use hydrolysis hydrothermal to prepare a TiO2 nanoparticle precursor solution, and use titanium Hydrolyze tetrabutyl ester in deionized water, then use the hydrothermal method to synthesize nanoparticles 200 with a particle size of 200-500nm, and disperse 1g of synthesized TiO2 particles into 0.05g of lauric acid, 0.2g of ethyl cellulose and 10ml After mixing the mixed solution of terpineol and ethanol (volume ratio 1:1), dilute it in absolute ethanol to make a TiSP precursor solution with a concentration of 5 mg / ml, and then take a piece of clean glass 100 and mix it in concentrated H2SO4 and Soak in H2O2 (volume ratio 3:1) mixed solution for 1.5 hours, then rinse with a large amount of deionized water and dry on a drying ...

Embodiment 3

[0044] according to figure 1One embodiment of the present invention shown is a method for capturing fetal nucleated red blood cells using a biocompatible TiO2 nanoparticle substrate. First, enter the preparation operation of TiO2 nanoparticles S1100: first use hydrolysis hydrothermal to prepare a TiO2 nanoparticle precursor solution, and use titanium Hydrolyze tetrabutyl ester in deionized water, then use the hydrothermal method to synthesize nanoparticles 200 with a particle size of 200-500nm, and disperse 1g of synthesized TiO2 particles into 0.05g of lauric acid, 0.2g of ethyl cellulose and 10ml After mixing the mixed solution of terpineol and ethanol (volume ratio 1:1), dilute it in absolute ethanol to make a TiSP precursor solution with a concentration of 5 mg / ml, and then take a piece of clean glass 100 and mix it in concentrated H2SO4 and H2O2 (Volume ratio is 3:1) Soak in the mixed solution for 1.5 hours, then rinse with a large amount of deionized water and dry on a d...

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Abstract

The invention discloses a biological compatible nano substrate-based capture method of rare cells in blood (including, but not be limited to circulating tumor cells, circulating fibrocytes, and fetal nucleated red blood cells). According to a preparation method, controllable hydrolysis and hydrothermal reaction are adopted, layered nano particles are synthesized via two-step synthesis, and a precursor solution is prepared; a glass substrate is subjected to spin coating so as to realized film forming, and a single layer nano substrate with excellent light transmittance performance is obtained via sintering; and the surface of the nano substrate is modified with an antibody with high specificity via a series of matured chemical modification processes. According to the biological compatible nano substrate material-based capture method, high efficiency capture of fetal nucleated red blood cells is realized via interaction of the nano substrate with cell surfaces enhanced by the rough surface of the nano substrate, and antigen antibody specific binding. Fetal umbilical cord blood is taken as a research object; the biological compatible nano substrate material-based capture method is developed based on cellular immunofluorescence staining; biological compatible nano particles are used for capturing and detection of the rare cells in blood; and a new through is provided for capturing of the rare cells in blood with nano materials. The biological compatible nano substrate material-based capture method possesses promising application potential in research of the rare cells in blood.

Description

Technical field: [0001] The invention belongs to the field of biological materials and relates to a method for capturing rare cells in blood based on a biocompatible nanoparticle substrate. Background technique: [0002] Fetal nucleated erythrocytes are a special sign of fetal hematopoietic system and fetal peripheral blood circulation, and are a rare cell in the peripheral blood of pregnant women. Fetal blood circulation is established at the end of 3 weeks after fertilization, and its red blood cells mainly come from the yolk sac. At term, 90% of red blood cells are derived from the bone marrow. Nucleated red blood cells not only exist in the blood circulation of the fetus, but also can enter the peripheral blood of the mother through the placental barrier, and its number gradually decreases with the increase of gestational age. Fetal nucleated erythrocytes have many characteristics: ①Nucleated erythrocytes do not exist in the peripheral blood of normal people, and if th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 石莹
Owner 石莹
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