Nano material capable of trapping erythrocytes

Abstract

The invention discloses a biological compatible nano substrate-based capture method of rare cells in blood (including, but not be limited to circulating tumor cells, circulating fibrocytes, and fetal nucleated red blood cells). According to a preparation method, controllable hydrolysis and hydrothermal reaction are adopted, layered nano particles are synthesized via two-step synthesis, and a precursor solution is prepared; a glass substrate is subjected to spin coating so as to realized film forming, and a single layer nano substrate with excellent light transmittance performance is obtained via sintering; and the surface of the nano substrate is modified with an antibody with high specificity via a series of matured chemical modification processes. According to the biological compatible nano substrate material-based capture method, high efficiency capture of fetal nucleated red blood cells is realized via interaction of the nano substrate with cell surfaces enhanced by the rough surface of the nano substrate, and antigen antibody specific binding. Fetal umbilical cord blood is taken as a research object; the biological compatible nano substrate material-based capture method is developed based on cellular immunofluorescence staining; biological compatible nano particles are used for capturing and detection of the rare cells in blood; and a new through is provided for capturing of the rare cells in blood with nano materials. The biological compatible nano substrate material-based capture method possesses promising application potential in research of the rare cells in blood.

Description

Technical field:

[0001] The invention belongs to the field of biological materials and relates to a method for capturing rare cells in blood based on a biocompatible nanoparticle substrate. Background technique:

[0002] Fetal nucleated erythrocytes are a special sign of fetal hematopoietic system and fetal peripheral blood circulation, and are a rare cell in the peripheral blood of pregnant women. Fetal blood circulation is established at the end of 3 weeks after fertilization, and its red blood cells mainly come from the yolk sac. At term, 90% of red blood cells are derived from the bone marrow. Nucleated red blood cells not only exist in the blood circulation of the fetus, but also can enter the peripheral blood of the mother through the placental barrier, and its number gradually decreases with the increase of gestational age. Fetal nucleated erythrocytes have many characteristics: ①Nucleated erythrocytes do not exist in the peripheral blood of normal people, and if th...

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