Method for obtaining treatment target causing skin keloids

A technology for keloids and therapeutic targets, applied in the fields of biomedical engineering and proteomics, can solve problems such as hindering diagnosis and treatment methods, and not understanding the mechanism of keloid formation.

Pending Publication Date: 2021-03-12
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, very limited understanding of the specific role of the ECM in pathological scar formation leads to a lack of understanding of the mechanism of keloid formation and hinders the development of new diagnostic and therapeutic approaches

Method used

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  • Method for obtaining treatment target causing skin keloids
  • Method for obtaining treatment target causing skin keloids
  • Method for obtaining treatment target causing skin keloids

Examples

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specific example

[0038] This example utilizes the method of the present invention to find out the therapeutic target of keloid, and the process is as follows figure 1 As shown, specifically: decellularize the keloid skin and the healthy skin to obtain the extracellular matrix scaffold. Through the matrix omics identification of the two groups of scaffolds, the differential components of protein expression between the two groups were obtained. Using bioinformatics tools and databases (DAVIDBioinformatics Resources 6.8) to carry out functional analysis and interaction network analysis on these differential components, it was found that proteins up-regulated and down-regulated in keloids, such as up-regulated protein SPARC and down-regulated protein MFAP4, these proteins may be responsible for the formation of Causes of keloids. It was found that the estrogen receptor pathway is a key pathway in the mechanism of scar formation, therefore, inhibition of estrogen receptor ERα or ERβ by inhibitors ...

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Abstract

The invention discloses a method for obtaining a treatment target causing skin keloids, and relates to the field of biomedical engineering and proteomics, and the method comprises the following steps:firstly, obtaining a skin matrix scaffold of human keloids; identifying the matrix protein component of the skin keloid skin by using tandem mass spectrometry; then comparing the keloid with matrix protein components of normal skin, and screening a keloid diagnostic marker; then carrying out immunofluorescence staining to confirm the specific mechanism of keloid formation; and finally, screeninghormone receptors serving as potential treatment targets of the keloids, and finding out the hormone receptors serving as the treatment targets causing the skin keloids through pathological staining and animal experiment verification. According to the method, by revealing the pathological mechanism of keloid formation, the diagnostic marker is identified, and the hormone receptor causing the skinkeloid is found out and serves as a treatment target.

Description

technical field [0001] The invention relates to the fields of biomedical engineering and proteomics, specifically a biomarker combination for diagnosing skin keloids and a new potential target for treating skin keloids. Background technique [0002] Consisting of the epidermis and dermis, the skin is the largest organ in the human body and plays an important role in protecting the body from external disturbances. Following skin injury, the wound healing process begins rapidly, involving inflammation, proliferation, and remodeling. While the ideal outcome for wound healing is scarless healing that resembles normal skin in color and texture, the best outcome is usually a flat, pliable scar with slight discoloration. However, abnormal wound healing can lead to pathological scars such as hypertrophic scars and keloids (1, Tredget, E.E., Levi, B., and Donelan, M.B. (2014) Biology and Principles of ScarManagement and Burn Reconstruction. Surg Clin North Am 94, 793-815; 2, Xue, M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N30/02G01N30/06G01N30/86G01N30/72G01N21/31
CPCG01N33/68G01N33/6887G01N33/74G01N33/5743G01N33/57484G01N30/02G01N30/06G01N30/8675G01N30/72G01N21/31G01N2333/72G01N2333/78
Inventor 冷泠
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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