Application of new kidney-warming sperm-producing drink as medicine for reducing expression level of H3K27me3

An expression-level, kidney-warming technology, applied in drug combinations, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problems of not finding the relationship between the expression levels of H3K27me3, not finding the use of Xinshen Shengjing Drink, etc. Increases growth rate and reduces developmental retardation

Inactive Publication Date: 2021-08-24
JINLIN MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the existing technology only uses Xin Wenshen Shengjing Decoction to treat sexual dysfunction and increase the incidence and maturation rate of sperm, but has not found the relationship between it and the expression level of H3K27me3, nor has it found that Xin Wenshen Shengjing Decoction Use in regulating H3K27me3 expression level

Method used

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  • Application of new kidney-warming sperm-producing drink as medicine for reducing expression level of H3K27me3
  • Application of new kidney-warming sperm-producing drink as medicine for reducing expression level of H3K27me3
  • Application of new kidney-warming sperm-producing drink as medicine for reducing expression level of H3K27me3

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Embodiment Construction

[0037] In order to enable those skilled in the art to better understand that the technical solutions of the present invention can be implemented, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0038] The methods used in the following examples are conventional methods unless otherwise specified, and all reagents used are commercially available.

[0039] 1 Materials and processing methods

[0040] 1.1 Test animals

[0041] Sexually mature Kunming strain 10-week-old male mice and 8-week-old female mice were used in the experiment. Mice lived in a temperature-controlled room with a 12h light / 12h dark photoperiod. Mice had free access to water and food. The treatment of mice was approved by the Ethics Committee of Jilin Medical University.

[0042] 1.2 Preparation of Xinwenshen Shengjing Drink

[0043]The compound Chinese medicine New Wenshen Shengjing Decoction (NWSSJD) contains ginseng 6g, Cynomoriu...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to application of a novel kidney-warming sperm-producing drink for promoting early embryonic development by maintaining the low level of H3K27me3. The method comprises the following steps: molding sexually mature male mice with cyclophosphamide, drenching the male mice with a new kidney-warming sperm-producing drink for 30 days, mating the treated male mice with female mice subjected to superovulation treatment, and counting the developmental rate of embryos in each stage; detecting the expression of H3K27me3 in sperms, prokaryotic embryos and 2-cell embryos by using an immunofluorescence staining method; detecting the expression of histone demethylase KDM6A and methylated transferase EZH2 in a 2-cell embryo with development retardation by using western blotting; using the qRT-PCR for analyzing the expression of zygote genome activation genes (ZSCAN4, E1F1AX, HSPA1A, ERV4-2 and MYC) in the 2-cell embryo with development retardation. According to the analysis of the results, the new application of the new kidney-warming sperm-producing drink for promoting early embryonic development by maintaining the low H3K27me3 modification level in sperms and male pronucleus is found.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of Xin Wenshen Shengjing Decoction as a medicine for reducing the expression level of H3K27me3. Background technique [0002] Xin Wenshen Shengjing Decoction is a traditional compound Chinese medicine, which is widely used in the treatment of male spermatogenesis disorders. New Wenshen Shengjing Decoction (New Wenshen Shengjing Decoction, NWSSJD) is composed of 15 kinds of traditional Chinese medicines, mainly including ginseng, antler, Cynomorium, astragalus, epimedium and angelica, etc. It can maintain high androgen concentration in testicular tissue, promote the development of germ cells at all levels, inhibit the apoptosis of germ cells, and increase the incidence and maturity of sperm. An important criterion for testing drugs for the treatment of male spermatogenic disorders is whether they can produce normal offspring. Therefore, studying the effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8945A61P15/08A61K35/32
CPCA61K36/258A61K36/185A61K36/481A61K36/296A61K35/32A61K36/64A61K36/232A61K36/8945A61K36/284A61K36/236A61K36/71A61K36/65A61K36/54A61K36/285A61K36/235A61P15/08
Inventor 潘晓燕雷钧涛孙艳美张万生王雪楠
Owner JINLIN MEDICAL COLLEGE
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