Method for detecting rare cells in blood based on surface enhancement effect

A rare cell, surface-enhanced technology, applied in the field of medical diagnosis, can solve the problems of false negatives and low capture efficiency of CTC cells, and achieve the effect of high capture efficiency, improved capture ability, and enhanced detection signal.
CN109060756AInactive Publication Date: 2018-12-21NINGBO MEIJING MEDICAL TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NINGBO MEIJING MEDICAL TECH
Publication Date
2018-12-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting rare cells in blood based on surface enhancement effect, which is characterized by comprising the steps of: plating a layer of plasma colored metal membrane on the surface of slide using spray coating method, and obtaining a SERS chip with metal nanostructure; arranging the SERS chip into a capture box of a capture apparatus; loading a pretreated blood sample in the capture apparatus, setting operating parameters of the capture apparatus, and capturing rare cells in the blood sample by an immune-magnetic enrichment method; taking the SERS chip with metal nanostructure out of the capture box after capturing, and performing immunofluorescence staining on the SERS chip after drying at 37 DEG C; and scanning and detecting the stained rare cells using a detection system. According to the method for detecting rare cells in blood based on surface enhancement effect, the capturing efficiency of rare cells such as CTC cells in the blood can be obviously improved, and the detection signal of rare cells can be enhanced
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Description

technical field

[0001] The invention relates to the field of medical diagnosis, in particular to a method for detecting rare cells in blood based on a surface enhancement effect. Background technique

[0002] Malignant tumors seriously threaten human health, and the main cause of death is that some tumor cells acquire the ability to invade and cause distant metastasis. Tumor metastasis is a complex process involving multi-step factors. Tumor cells with metastatic potential break away from the primary tumor and metastasize to the blood, bone marrow, lymph nodes or distant organs in very small numbers, which are difficult to detect using conventional detection methods. for tumor micrometastases. The shedding, invasion and entry of tumor cells into the blood circulation are the initial stages of tumor metastasis, and provide the possibility for the formation of tumor metastases. Therefore, the detection of circulating tumor cells (CTC) in peripheral blood has important clinica...

Claims

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