The invention relates to a method for preparing a pancreatic stone protein antibody and a method for detecting PSP (pancreatic stone protein), and belongs to the technical field of gene recombinationof bioengineering. The invention aims to provide the method for preparing the pancreatic stone protein antibody and the method for detecting PSP (pancreatic stone protein), so that a specific PSP immunogen is prepared, a PSP monoclonal antibody or polyclonal antibody is produced, the PSP detecting method is established, and a PSP in vitro diagnosis reagent kit is established. A tag-PSP recombinantprotein carrier is constructed through a gene recombination technique, a tag-PSP recombinant protein is expressed by bacteria, yeast, insect cells, animal cells or human cells, and the tag-PSP recombinant protein is purified to be used as a PSP recombinant protein immunogen. The method disclosed by the invention is used for detecting rise of the level of the PSP in blood, and can provide a reagent kit which can diagnose the generation of bacterial septicemia early, quickly and accurately, guide treatment of septicemia, monitor the progress of the septicemia and quickly detect the PSP by an immunology method.