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Gamma synuclein colloidal gold detection device and preparation method and application thereof

A synuclein and detection device technology, applied in the field of medical detection, can solve the problems of time-consuming, cumbersome ELISA detection process, and inability to realize bedside detection, and achieve the effect of low detection cost and important clinical value

Pending Publication Date: 2019-03-19
BIOCHAIN BEIJING SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, there is no detection method for SNCG in the field of clinical detection. In the field of patents and scientific research literature, there are reports on the use of ELISA technology platform to detect SNCG. However, the ELISA detection process is cumbersome and time-consuming, and bedside detection (POCT) cannot be realized.

Method used

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  • Gamma synuclein colloidal gold detection device and preparation method and application thereof
  • Gamma synuclein colloidal gold detection device and preparation method and application thereof
  • Gamma synuclein colloidal gold detection device and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The preparation of embodiment 1 SNCG colloidal gold kit

[0067] 1. Liquid preparation

[0068] 1.1 Preparation of 10% chloroauric acid solution:

[0069] Take 1.0g of chloroauric acid, equilibrate to room temperature, measure 2.0mL of purified water, put it into a glass bottle containing chloroauric acid, and blow repeatedly until the chloroauric acid is completely dissolved. Pour the dissolved auric acid into the container, rinse the glass bottle of auric acid with purified water for 5 times, and pour the washing solution back into the container. The solution was made up to 10.0 mL with purified water. Cap the bottle tightly and shake well for 15 minutes to mix well.

[0070] 1.2 Preparation of gold conjugate resuspension:

[0071] Weigh 6.06g of Tris, 50.0g of sucrose, 5.00 of bovine serum albumin, 10g of PEG20000, 5.00mL of Tween 20, 1ml of PC300, add 700.0mL of purified water, stir to fully dissolve, add an appropriate amount of concentrated hydrochloric acid...

Embodiment 2

[0090] Example 2 Sodium caseinate and BSA are compared as the influence of stabilizer on performance

[0091] Use the kit prepared in Example 1 (sodium caseinate as a stabilizer) and the control kit (10% BSA as a stabilizer, all the other conditions are the same as in Example 1) to simultaneously detect 10 cases of bladder cancer, 10 cases of renal cancer, and 10 cases of prostate cancer Cancer, 10 cases of endometrial cancer, 10 cases of breast cancer patients before treatment urine samples and 10 cases of normal urine samples (all obtained from Boercheng (Beijing) Technology Co., Ltd.), compared two different preparations The sensitivity and specificity of the kit of the process are tested according to the following methods:

[0092] 1) Take out the test card and place it flat on a plane. In this embodiment, the test card used is the card kit prepared in Example 1;

[0093] 2) Use a dropper to absorb urine and add 2 to 3 drops to the sample hole S of the test card;

[00...

Embodiment 3

[0102] Example 3 Detection of SNCG in Bladder Cancer and Normal Healthy Person Samples

[0103] Urine samples from 25 cases of bladder cancer before treatment (all obtained from Boercheng (Beijing) Technology Co., Ltd.) and 54 normal people’s urine samples (both obtained from Boercheng (Beijing) Technology Co., Ltd.) were used as test objects , complete the detection as follows:

[0104] 1) Take out the test card and place it flat on a plane. In this embodiment, the test card used is the card kit prepared in Example 1;

[0105] 2) Use a dropper to absorb urine and add 2 to 3 drops to the sample hole S of the test card;

[0106] 3) 10 minutes after adding the sample, refer to the following standards for result interpretation (see figure 2 shown):

[0107] a) Positive: a red band appears in both the detection area (T) and the quality control area (C);

[0108] b) Negative: Only a red band appears in the quality control area (C), and no red band appears in the detection ar...

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Abstract

A gamma synuclein colloidal gold detection device comprises a strip base material. A sample pad, a colloidal gold conjugate pad, a reaction film and filter paper are sequentially arranged on the stripbase material. The colloidal gold conjugate pad is coated with colloidal gold marked rat anti-gamma synuclein monoclonal antibody alpha SNCG2; rat anti-gamma synuclein monoclonal antibody alpha SNCG1is sprayed to a testing area (T) of the reaction film; goat anti-mouse immune globulin IgG is sprayed to a quality control area (C). The colloidal gold detection device takes sodium casein as a stabilizing agent. The invention provides a preparation method and application of the gamma synuclein colloidal gold detection device. The gamma synuclein colloidal gold detection device has advantages that no other experimental equipment is needed in a detection process, low detection cost is realized, and a significant clinical value to diagnosis of carcinoma of urinary bladder, kidney cancer, prostatic cancer, endometrial cancer and breast cancer is achieved.

Description

technical field [0001] The invention belongs to the field of medical detection. Specifically, the invention relates to a colloidal gold detection device for detecting γ-synuclein (SNCG), and the invention also relates to a preparation method of the device and its use in preparing a disease detection device use. Background technique [0002] In recent years, malignant tumors have become one of the major diseases that seriously endanger human health and life worldwide. People talk about the discoloration of cancer, and generally think that cancer is an incurable disease, which is mainly due to its high cure rate, high mortality rate and wide distribution. Statistics show that in 2015, there were 4.292 million new cancer cases and 2.814 million deaths in my country. The incidence of cancer in my country is close to the world level, but the mortality rate is higher than the world level. [0003] An important reason for the high mortality rate of cancer is that most cancers in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/543
CPCG01N33/54306G01N33/54313G01N33/5748
Inventor 荆志强周光朋韩晓亮王建铭
Owner BIOCHAIN BEIJING SCI & TECH
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