Composite nano colloidal gold chitosan immune carrier and preparation method thereof

A nano-colloid, chitosan technology, applied in chemical instruments and methods, other chemical processes, instruments, etc., can solve the problems of limited adsorption capacity, increased colloidal gold dosage, and increased cost.

Inactive Publication Date: 2012-05-02
JIANGSU YITONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limited ability of colloidal gold to adsorb antigens and antibodies, sometimes in order to achieve a certain sensitivity, the amount of colloidal gold should be increased, which greatly increases the cost and wastes precious gold resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Follow the steps below to prepare the composite nanocolloidal gold chitosan carrier

[0023] (1) Preparation of colloidal gold: Take 100mL chloroauric acid aqueous solution with a mass concentration of 0.01% and heat it to boiling, add 4mL of a preheated 1% trisodium citrate aqueous solution with mass concentration under stirring, and the golden yellow chloroauric acid aqueous solution will change within 2 minutes. It turns gray and then turns from gray to wine red. Continue to stir and boil until the solution turns bright red. After cooling, dilute to 100 mL with double distilled water, and store in a refrigerator at 4°C in the dark;

[0024] (2) Preparation of chitosan solution: Dissolve chitosan with 0.1-1% citric acid into 0.1% chitosan solution, dissolve overnight and filter to obtain a transparent chitosan solution;

[0025] (3) Preparation of composite nano colloidal gold chitosan immune carrier: mix the above colloidal gold and chitosan solution at a mass ratio ...

Embodiment 2

[0027] Follow the steps below to prepare the composite nanocolloidal gold chitosan carrier

[0028] (1) Preparation of colloidal gold: Take 100mL chloroauric acid aqueous solution with a mass concentration of 0.01% and heat it to boiling, add 4mL of a preheated 1% trisodium citrate aqueous solution with mass concentration under stirring, and the golden yellow chloroauric acid aqueous solution will change within 2 minutes. It turns gray and then turns from gray to wine red. Continue to stir and boil until the solution turns bright red. After cooling, dilute to 100 mL with double distilled water, and store in a refrigerator at 4°C in the dark;

[0029] (2) Preparation of chitosan solution: Dissolve chitosan with a deacetylation degree greater than 90% into 0.01% chitosan solution with 0.1-1% citric acid, dissolve overnight and filter to obtain transparent chitosan solution;

[0030] (3) Preparation of composite nano colloidal gold chitosan immune carrier: mix the above colloida...

Embodiment 3

[0032] (1) Preparation of colloidal gold: Take 100mL chloroauric acid aqueous solution with a mass concentration of 0.01% and heat it to boiling, add 4mL of a preheated 1% trisodium citrate aqueous solution with mass concentration under stirring, and the golden yellow chloroauric acid aqueous solution will change within 2 minutes. It turns gray and then turns from gray to wine red. Continue to stir and boil until the solution turns bright red. After cooling, dilute to 100 mL with double distilled water, and store in a refrigerator at 4°C in the dark;

[0033] (2) Preparation of chitosan solution: Dissolve chitosan with a deacetylation degree greater than 90% into 0.1% chitosan solution with 0.1-1% citric acid, dissolve overnight and filter to obtain transparent chitosan solution;

[0034] (3) Preparation of composite nano colloidal gold chitosan immune carrier: mix the above colloidal gold and chitosan solution at a mass ratio of 1:0.5, then slowly add 0.05mol / L potassium carb...

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Abstract

The invention provides a composite nano colloidal gold chitosan immune carrier, wherein, colloidal gold is uniformly dispersed in chitosan. The invention also provides a preparation method of the composite nano colloidal gold chitosan immune carrier and a labeled antigen-antibody. According to the invention, the characteristic that chitosan has good affinity for the antigen and antibody is applied in the colloidal gold, thus the adsorption capability of the colloidal gold for the antigen-antibody is increased, and the amount of the colloidal gold is reduced. The composite nano colloidal gold chitosan immune carrier can be widely used for immunodetection or immunodiagnosis in the fields of biomedicine or agriculture and the like.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a composite nano colloidal gold chitosan carrier and its preparation method and application. Background technique [0002] Colloidal gold immunochromatography is a rapid diagnostic technology based on immunocolloidal gold technology that emerged in the United States in the 1990s. It is a new type of detection developed on the basis of modern monoclonal antibody technology, colloidal gold immune technology and new material technology. technology. Colloidal gold solution refers to the gold sol whose dispersed phase particle diameter is between 1 and 150 nm, which belongs to heterogeneous heterogeneous system, and the color is orange-red to purple-red. [0003] In 1971, Faulk and Taylor introduced colloidal gold into immunochemistry and began to use it as an immunolabeling technique. The application of colloidal gold in immunochemistry is also called immunogold. In recent years,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30G01N33/543
Inventor 张添杨宇民
Owner JIANGSU YITONG BIOTECHNOLOGY CO LTD
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