SARS-CoV-2 neutralizing antibody detection kit

An antibody detection, sars-cov-2 technology, applied in the field of biomedical detection, can solve the problems of cumbersome operation, narrow detection range, poor sensitivity, etc., and achieve the effect of good repeatability of results, few interference factors, and short detection time.

Pending Publication Date: 2020-08-21
威海威高生物科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The detection methods of the new coronavirus mainly include nucleic acid detection and antibody detection. Nucleic acid detection requires high standards for instruments, and the operation is cumbersome, time-consuming, and expensive. Antibody detection methods include magnetic particle chemiluminescence, colloidal gold, and ordinary chemiluminescence. Ordinary chemiluminescence method takes a long time to dete

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The invention provides a novel coronavirus neutralizing antibody magnetic particle chemiluminescence detection kit and a preparation method, the specific steps are as follows:

[0058] 1) The novel coronavirus S protein antigen labeled with magnetic particles, including the preparation of magnetic particle preservation solution;

[0059] 2) The novel coronavirus S protein antigen labeled with acridinium ester, including the preparation of diluent;

[0060] 3) Preparation of novel coronavirus neutralizing antibody calibrator 1;

[0061] 4) Preparation of novel coronavirus neutralizing antibody calibrator 2.

[0062] Other materials that may be needed: Substrate A solution for automatic immunoassay system, Substrate B solution for automatic immunoassay system, cleaning solution, cuvette, purified water required for the experiment process.

[0063] The preparation of the magnetic particle preservation solution in the step 1) comprises the following steps:

[0064] 1.1 W...

Embodiment 2

[0097] A preparation method of a novel coronavirus neutralizing antibody rapid diagnostic kit, the cartridge of the kit contains a novel coronavirus neutralizing antibody detection test strip, and the test strip is composed of a sample pad, a gold standard pad, a reaction membrane and the water-absorbing layer are pasted on it in turn, the test strip is made of glass fiber as the sample pad, the absorbent paper is used as the water-absorbing layer, and the paper card with adhesion is used as the backing card; the gold standard pad is a new type of coronavirus antigen labeled with colloidal gold. Loaded on a glass fiber mat, the reaction membrane is made by coating a new coronavirus S protein antigen on a nitrocellulose membrane to make a detection line, and at the same time coated with a mouse anti-IgG antibody to make a quality control line. The specific preparation method includes the following steps :

[0098] 1. Preparation of Gold Label Pads

[0099] It is to load the co...

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Abstract

The invention relates to an SARS-CoV-2 neutralizing antibody detection kit. The SARS-CoV-2 neutralizing antibody detection kit comprises a solid phase carrier, a first antigen and a second antigen ofS protein of SARS-CoV-2, and the second antigen is marked with a signal substance. Whether a tested person is infected by the new coronavirus or not and whether infection risks exist or not are judgedby detecting a neutralizing antibody through an immunodiagnosis technology, and the method is reliable in theory, practical and feasible and can be completed only in a secondary biosafety laboratory.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a SARS-CoV-2 neutralizing antibody detection kit. Background technique [0002] Coronaviridae is divided into 4 genera α, β, γ and δ, and the α and β genera coronaviruses can infect mammals and humans. Severe acute respiratory syndrome coronavirus 2 (severe acute respiratory syndrome coro-navirus 2, SARS-CoV-2) is a new type of coronavirus belonging to the genus β, round or oval, with a diameter of 60-140nm, and a crown-like shape under an electron microscope. Similar to SARS-CoV that emerged in 2002, SARS-CoV-2 belongs to the subgenus Sarbecovirus (Coronaviridae, Coronaviridae), and both rely on the ACE2 protein on the surface of human cells as a cell entry receptor. Protein sequence analysis found that the amino acid similarity between SARS-CoV-2 and SARS-CoV was 94.6% in seven conserved non-structural proteins. [0003] The existing detection methods for SARS-CoV-2 mainly...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/56983G01N33/54326G01N33/543G01N2333/165
Inventor 姚继承吴晗琪赵海英刘海光衣晓娜丛向明
Owner 威海威高生物科技有限公司
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