Magnetic particle chemiluminescence detection kit of free thyroxine and application thereof

A free thyroxine and kit technology, applied in the field of immunodiagnosis, can solve the problems of low sensitivity of enzyme immunoassay, complicated operation of radioimmunoassay, narrow monitoring range, etc., and achieve the effects of easy solid-liquid separation, improved sensitivity and reduced production cost.

Inactive Publication Date: 2009-10-07
天津九鼎医学生物工程有限公司 +1
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  • Abstract
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Problems solved by technology

[0003] For a long time, enzyme immunoassay or radioimmunoassay has been generally used in clinic to detect free thyroxine fT in patients. 4 However, these two methods have their disadvantages: for example, the operation of radioimmunoassay is complicated, the time is long, and there is a problem of radioactive contamination; the sensitivity of enzyme immunoassay is low and the monitoring range is narrow

Method used

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  • Magnetic particle chemiluminescence detection kit of free thyroxine and application thereof
  • Magnetic particle chemiluminescence detection kit of free thyroxine and application thereof
  • Magnetic particle chemiluminescence detection kit of free thyroxine and application thereof

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Embodiment Construction

[0025] The following specific examples are combined with the free thyroxine (fT 4 ) Quantitative detection kits and detection methods are further explained.

[0026] (1) Materials and instruments

[0027] 1. The concentrations of the standard products are 0pmol / L, 1pmol / L, 2.5pmol / L, 5pmol / L, 10pmol / L, 25pmol / L, 85pmol / L, 0.6ml each

[0028] 2. Magnetic bead separator (3ug / ml) 10ml

[0029] 3. 100ml lotion

[0030] 4. Luminescent substrate solution A and B solution 6ml each

[0031] 5. Horseradish peroxidase (HRP) labeled free thyroxine (fT 4 ) Antigen 10ml

[0032] 6. Antibody 10ml

[0033] 7. Specimen to be tested

[0034] 8. Semi-automatic tube chemiluminescence analyzer (Beijing Hamamatsu Photonics Technology Co., Ltd. BHP9507 chemiluminescence immunoassay analyzer)

[0035] 9. Water bath (for incubation at 37℃)

[0036] (2) Reagent preparation

[0037]The free thyroxine (fT 4 ) The standard is to use the phosphate buffer of pH 7.35-7.45 as the matrix of the calibrator, and th...

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Abstract

The present invention discloses a quantitative detection kit of free thyroxine (fT4) and a method for detecting the free thyroxine with the detection kit. The detection kit comprises a free thyroxine standard, a free thyroxine antigen marked by horseradish peroxidase, an antibody, a magnetic bead separating agent, luminescence substrate reagent and wash liquid. The method for detecting free thyroxine comprises the following steps: adding the free thyroxine standard and sample to be measured into the test tube, adding the free thyroxine antigen marked by horseradish peroxidase and antibody, after mixing to uniform, incubating for one hour in 37 DEG C, then adding magnetic bead separating agent for stewing for 5 minutes, separating for obtaining the immune magnetic microspheres, discarding the supernate, washing the deposit for two times; adding the luminescence substrate reagent, measuring and analyzing the concentration of free thyroxine in sample to be measured. The quantitative detection kit of free thyroxine and the detection method provided by the invention have the advantages of higher stability, higher sensitivity and higher accuracy, and belong to the technical field of immunodiagnosis.

Description

Technical field [0001] The invention belongs to the technical field of immunodiagnosis, and specifically relates to a free thyroxine fT 4 Quantitative detection kit, the present invention also relates to a method for detecting free thyroxine using the above kit. Background technique [0002] Thyroxine is a hormone synthesized and secreted by thyroid follicular cells. Thyroxine T produced by the thyroid 4 After being transported to the blood circulation, most of it is combined with protein and part of thyroxine T 4 No biological activity, its level cannot reflect the functional state of the thyroid, only about 0.03% of thyroxine T 4 Exist in the blood in a free state, free thyroxine fT 4 It is the active part of thyroid hormone in the circulating blood. It has biological activity. 4 It remains relatively constant in the blood and maintains its normal physiological functions, so free thyroxine fT 4 The level can accurately reflect the state of thyroid function, it is not affected b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/76
Inventor 温雷
Owner 天津九鼎医学生物工程有限公司
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