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Electropolymerization molecular imprinting technology-based double-parameter composite micro-sensor and preparation thereof

A molecular imprinting technology and micro-sensor technology, applied in the field of dual-parameter composite micro-sensor and preparation, can solve the problems of large equipment, high cost and high energy consumption

Inactive Publication Date: 2013-01-30
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, MEMS technology is becoming more and more mature, and has the technical conditions to transform the traditional molecular imprinting detection method from large equipment, high cost and high energy consumption to miniaturization, low cost and low energy consumption.

Method used

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  • Electropolymerization molecular imprinting technology-based double-parameter composite micro-sensor and preparation thereof
  • Electropolymerization molecular imprinting technology-based double-parameter composite micro-sensor and preparation thereof
  • Electropolymerization molecular imprinting technology-based double-parameter composite micro-sensor and preparation thereof

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Embodiment

[0064] A creatinine / urea composite microsensor based on electropolymerization molecular imprinting of the present invention. Such as figure 1 , figure 2 , image 3 As shown, the implementation steps are as follows:

[0065]1. First choose silicon wafer or glass as the substrate (1);

[0066] 2. Growing a silicon dioxide insulating layer (2) on the surface of the silicon wafer (as glass is not required);

[0067] 3. Based on the Lift-off technology, the counter electrode (3) and the reference electrode (4) are formed by photolithography and sputtering Pt thin film 400nm technology;

[0068] 4. Based on the Lift-off technology, the working electrode (5) is formed by photolithography and sputtering Au thin film 400nm;

[0069] 5. Based on MEMS technology, SU8 glue is used to cast a three-dimensional ring (7) with a height of 30 μm on the silicon dioxide insulating layer of the chip, which surrounds the working electrode and the counter electrode to form a micro-reaction poo...

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Abstract

Relating to sensors and molecular imprinting technologies, the invention discloses an electropolymerization molecular imprinting technology-based double-parameter composite micro-sensor and a preparation thereof. According to the invention, three electrochemical microelectrode systems are integrated on a same chip, and each electrochemical microelectrode system has its independent micro-electrochemical reaction pool. Through encapsulation by a sealant, the integrated chip can form an open composite measurement pool containing three electrochemical microelectrode systems. In the micro-reaction pool of each electrochemical microelectrode system, by injecting a solution from the outside, a molecular imprinting procedure containing in situ polymerization and ultrasonic elution of template molecules can be implemented separately, thus obtaining a molecularly imprinted sensor. The left and right microelectrode systems of the composite micro-sensor respectively recognize two corresponding molecules due to different molecularly imprinted sensitive membranes, and the electrochemical microelectrode system positioned in the middle is used as a differential detection reference so as to deduct background signals and environmental effects of a test system. The composite micro-sensor provided in the invention can recognize two molecules simultaneously.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors and molecular imprinting, and relates to a dual-parameter composite microsensor based on electropolymerization molecular imprinting and a preparation method thereof. Background technique [0002] With the rapid development of modern electronics and biotechnology, biosensing technology has formed an independent emerging high-tech field. Biosensors based on MEMS technology have the advantages of miniaturization, integration, and intelligence, and its supporting instruments have the advantages of portability, digitization, and ease of use. The effective and rapid detection characteristics of biosensors will bring new technological revolutions in the fields of biomedicine, environmental detection, food, and pharmaceutical industries. [0003] Imprinting technology is one of the three major discoveries in the field of molecular biology in the 20th century. Together with the discovery ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 夏善红任振兴韩泾鸿边超卞贺明
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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