Nucleoside components separated and purified from marine low-value shellfish and their preparation method and application
A low-value shellfish, separation and purification technology, applied in the field of marine low-value shellfish, can solve the problems of low purity, long production cycle, complicated separation process steps, etc., and achieves reasonable process design, short production cycle, and strong operability. Effect
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Embodiment 1
[0030] A method for separating and purifying nucleoside components from marine low-value shellfish, comprising the following steps:
[0031] (1) Preparation of the alcohol-precipitated supernatant of four-cornered clam: take fresh four-cornered clam soft body, wash and drain, weigh, add 3 times the amount of water to decoct, heat and reflux twice, each time for 45 minutes, filter, and combine the filtrate , concentrated, centrifuged to remove the precipitate, to obtain the supernatant, add ethanol to make the ethanol concentration 80%, let it stand overnight, and suction filter to obtain the supernatant; the supernatant is concentrated until it has no alcohol smell, and is desalted by electrodialysis with a voltage of 30V , the loading concentration is 30g (solid content) / L, and the desalination time is 1.5h.
[0032] (2) Coarse separation of macroporous adsorption resin column: take 1.6 times column volume of the four-cornered clam soft body water extraction alcohol precipita...
Embodiment 2
[0040] A method for isolating and purifying nucleoside components from clam philippines, comprising the following steps:
[0041] (1) Preparation of the ethanol-precipitated supernatant of Philippine clam: take fresh Philippine clam software, wash and drain, weigh, add 3 times the amount of water, heat and reflux twice, each time for 60 minutes, filter, and combine the filtrate , concentrated, centrifuged to remove the precipitate, to obtain a supernatant, added ethanol to make the ethanol concentration 75%, allowed to stand overnight, and filtered to obtain a supernatant. The supernatant was concentrated until there was no alcohol smell, and desalted by electrodialysis with a voltage of 20V, a sample concentration of 20g (solid content) / L, and a desalting time of 2h.
[0042](2) Coarse separation by macroporous adsorption resin column: take 2 times the column volume of the supernatant of philippine clam software prepared in step (1) by water extraction and ethanol precipitati...
Embodiment 3
[0050] A method for separating and purifying nucleoside components from cockles, comprising the following steps:
[0051] (1) Preparation of ethanol-precipitated supernatant of cockles: take fresh cockles soft body, wash and drain, weigh, add 4 times the amount of water, heat and reflux twice, 50min each time, filter, combine filtrates, concentrate, and centrifuge Remove the precipitate to obtain a supernatant, add ethanol to make the ethanol concentration 80%, let stand overnight, and filter with suction to obtain a supernatant. The supernatant was concentrated until it had no alcohol smell, and was desalted by electrodialysis with a voltage of 30V, a sample concentration of 34g (solid content) / L, and a desalination time of 1.5h.
[0052] (2) Coarse separation by macroporous adsorption resin column: take 1.5 times the column volume of the water-extracted and ethanol-precipitated supernatant of the clam software prepared in step (1), put it on the SP207 type macroporous adsorp...
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