Method for preparing heavy metal high-efficiency adsorbent by utilizing compound bacterial agent

A technology of heavy metals and compound bacteria, which is applied in the field of water treatment, can solve the problems of low adsorption efficiency and achieve the effects of high adsorption efficiency, stable performance, and strong bacterial vitality

Active Publication Date: 2018-01-12
北京美胜同创科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low adsorption efficiency of heavy metal adsorbents prepared by exopolysaccharides secreted by microorganisms at the present stage, the purpose of the present invention is to provide a method for preparing heavy metal high-efficiency adsorbents using a compound bacterial agent. The compound bacterial agent is marine halomonas Bacterial strain Halomonas sp.GHF11 and Psychrobacter sp.GHF2, both strains were isolated and purified from the sludge spit out by clam philippines, and the two strains were used in combination to strengthen each other during the fermentation process. The produced exopolysaccharide has high yield and good adsorption effect, and the obtained adsorbent has high adsorption efficiency and stable performance

Method used

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  • Method for preparing heavy metal high-efficiency adsorbent by utilizing compound bacterial agent
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  • Method for preparing heavy metal high-efficiency adsorbent by utilizing compound bacterial agent

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Embodiment 1

[0042] A method for preparing a heavy metal high-efficiency adsorbent by using a compound bacterial agent, comprising the following steps:

[0043] (1) Inoculate the strains of Halomonas sp.GHF11 and Psychrobactersp.GHF2 on solid medium for 36 hours. The culture temperature of Halomonas sp. The culture temperature of Pyrobacterium strain is 18°C, and then add sterile water to make GHF11 strain liquid and GHF2 strain liquid respectively, and the volume ratio of sterile water to solid medium is 1:1;

[0044] (2) Mix the GHF11 strain solution with the GHF2 strain solution in a volume ratio of 0.5:1 to obtain a uniform mixture, then inoculate it into a liquid medium, and the inoculation concentration of the mixed bacteria solution is 1.5mL mixed bacteria solution / 100mL liquid medium, Ferment and cultivate at 23°C for 3 days, and the shaker rotates at a speed of 100r / min to make a fermentation broth;

[0045] (3) Dialyze the fermented bacteria liquid to remove part of the medium c...

Embodiment 2

[0055] A method for preparing a heavy metal high-efficiency adsorbent by using a compound bacterial agent, comprising the following steps:

[0056] (1) Inoculate the strains of Halomonas sp.GHF11 and Psychrobactersp.GHF2 on solid medium for 42 hours, and culture the Halomonas strains at 28°C. The culture temperature of Pyrobacterium strain is 20°C, and then add sterile water to make GHF11 strain liquid and GHF2 strain liquid respectively, the volume ratio of sterile water to solid medium is 1.25:1;

[0057] (2) Mix the GHF11 bacterial seed liquid and the GHF2 bacterial seed liquid in a volume ratio of 1:1 to obtain a uniform mixture, then inoculate into the liquid medium, the inoculation concentration of the mixed bacterial liquid is 2mL mixed bacterial liquid / 100mL liquid medium, in Ferment and cultivate at 25°C for 4 days, and the shaker rotates at 130r / min to make a fermentation broth;

[0058] (3) Dialyze the fermented bacteria liquid to remove part of the medium componen...

Embodiment 3

[0068] A method for preparing a heavy metal high-efficiency adsorbent by using a compound bacterial agent, comprising the following steps:

[0069] (1) Inoculate the strains of Halomonas sp.GHF11 and Psychrobactersp.GHF2 on solid medium for 48 hours. The culture temperature of Halomonas sp. The culture temperature of Pyrobacterium strain is 23°C, and then add sterile water to make GHF11 strain liquid and GHF2 strain liquid, the volume ratio of sterile water to solid medium is 1.5:1;

[0070] (2) Mix the GHF11 strain solution with the GHF2 strain solution in a volume ratio of 2:1 to obtain a uniform mix, then inoculate it into a liquid medium, and the inoculation concentration of the mixed bacteria solution is 2.5 mL mixed bacteria solution / 100 mL liquid medium, Ferment and cultivate at 27°C for 5 days, and the shaking table rotates at a speed of 160r / min to make a fermentation broth;

[0071] (3) Dialyze the fermented bacteria liquid to remove part of the medium components an...

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Abstract

The invention relates to the technical field of water treatment, and in particular relates to a method for preparing a heavy metal high-efficiency adsorbent by utilizing a compound bacterial agent. The compound bacterial agent comprises marine salt monoucleosis strains Halomonas sp.GHF11 and marine psychrobacter psychrophilus strains Psychrobacter sp .GHF2; the two types of strains are both obtained by separating and purifying sludge liquid which is spit by ruditapes philippinarum; the two types of the strains are compounded and fermented after being subjected to enlarged cultivation respectively; the two types of the strains are synergic to strengthen each other in fermenting and cultivating processes; the activity of the strains is high; the yield of produced exopolysaccharide is high; the exopolysaccharide is separated by dialyzing a fermented fermenting bacteria liquid, removing part of culture medium components, performing centrifuging and performing ethanol precipitation twice, and then is prepared into the heavy metal adsorbent by curing through agar and starch; the heavy metal adsorbent is good in adsorption effect, high in adsorption efficiency and stable in performance, is safe and harmless, and can be directly added into drinking water.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a method for preparing a heavy metal high-efficiency adsorbent by using a compound bacterial agent. Background technique [0002] Most of the water used by people in production and life is tap water, which is mainly obtained by treating water drawn from groundwater or river water by water plants. Because some water sources are affected by industrial or domestic sewage discharges, the heavy metal content exceeds the standard. Although it is treated by a water plant before being used for human and livestock consumption, the content of some heavy metals is still at a high level, and some even exceed the standard; in addition, urban tap water pipelines Old age will also cause heavy metals such as lead to infiltrate into the water body and cause heavy metal content to exceed the standard; although there are water purifiers with heavy metal removal function on the market, the w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P1/04B01J20/24B01J20/30C02F1/28C12R1/01C02F101/20
Inventor 穆军穆思源崔霞
Owner 北京美胜同创科技发展有限公司
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