Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparation of heavy metal adsorbent from marine Psychrobacter strain

A heavy metal and adsorbent technology, applied in the field of water treatment, can solve the problems of low adsorption efficiency of adsorbents and high adsorption efficiency of heavy metal adsorbents, and achieve the effects of stable performance, strong adsorption capacity and high adsorption efficiency

Active Publication Date: 2018-01-26
ZHEJIANG OCEAN UNIV
View PDF5 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low adsorption efficiency of adsorbents prepared by secreting exopolysaccharides from existing microorganisms, the object of the present invention is to provide a method for preparing heavy metal adsorbents using Pyrobacter sp. GHF2 is isolated from the sludge liquid spit out by clams. The exopolysaccharide secreted by this strain has a large specific surface area, rich complexing groups and strong negative charge, and the ability to adsorb heavy metals is stable. The adsorption efficiency of the adsorbent is high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparation of heavy metal adsorbent from marine Psychrobacter strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing a heavy metal adsorbent by utilizing marine Pyrobacterium strains, comprising the following steps:

[0032] (1) Inoculate the selected strain of Psychrobacter sp.GHF2 on a solid medium for 36 hours at 18°C, add sterile water to make a strain solution, and the volume ratio of sterile water to solid medium is 1.0 :1;

[0033] (2) Inoculate the seed liquid into the liquid medium, the inoculation concentration is 0.8mL of the seed liquid / 100mL liquid medium, ferment and cultivate at 18°C ​​for 3 days, and the shaker speed is 160r / min, to prepare the fermented bacterial liquid;

[0034] (3) Dialyze the fermented bacteria liquid to remove part of the medium components and centrifuge to obtain the supernatant. The specific process is: place the fermented bacterial liquid on a rotary evaporator, concentrate under reduced pressure at 60°C to 10% of the original volume, and then Put it into a dialysis bag with a molecular weight cut-off of 8000 Daltons and...

Embodiment 2

[0039] A method for preparing a heavy metal adsorbent by utilizing marine Pyrobacterium strains, comprising the following steps:

[0040] (1) Inoculate the selected strains of Psychrobacter sp.GHF2 onto a solid medium, culture at 20°C for 42 hours, add sterile water to make a strain solution, and the volume ratio of sterile water to solid medium is 1.25:1;

[0041] (2) Inoculate the seed liquid into the liquid medium, the inoculation concentration is 1.1mL of the seed liquid / 100mL of the liquid medium, ferment and cultivate at 20°C for 4 days, and the shaker speed is 180r / min, to prepare the fermented bacterial liquid;

[0042] (3) Dialyze the fermented bacteria liquid to remove part of the medium components and then centrifuge to obtain the supernatant. The specific process is: place the fermented bacteria liquid on a rotary evaporator, concentrate under reduced pressure at 62°C to 12% of the original volume, and then Put it into a dialysis bag with a molecular weight cut-of...

Embodiment 3

[0047] A method for preparing a heavy metal adsorbent by utilizing marine Pyrobacterium strains, comprising the following steps:

[0048] (1) Inoculate the selected strains of Psychrobacter sp.GHF2 onto a solid medium, culture at 23°C for 48 hours, add sterile water to make a strain solution, and the volume ratio of sterile water to solid medium is 1.5:1;

[0049] (2) Inoculate the seed liquid into the liquid medium, the inoculation concentration is 1.4mL of the seed liquid / 100mL of the liquid medium, ferment and cultivate at 23°C for 5 days, and the shaker speed is 200r / min, to prepare the fermented bacterial liquid;

[0050] (3) Dialyze the fermented bacteria liquid to remove part of the medium components and then centrifuge to obtain the supernatant. The specific process is: place the fermented bacterial liquid on a rotary evaporator, concentrate under reduced pressure at 65°C to 15% of the original volume, and then Put it into a dialysis bag with a molecular weight cut-of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of water treatment, in particular to a method for preparation of a heavy metal adsorbent from a marine Psychrobacter strain. The selected marine Psychrobacter strain Psychrobacter sp.GHF2 is separated from the sludge liquid spit by ruditapes philippinarum, the strain is subjected to enlarged culture to prepare a strain solution, and fermentation cultureis carried out to produce abundant exopolysaccharide, then the fermented strain solution is dialyzed to remove part of medium components, and centrifugation is conducted to obtain supernatant, ethanol precipitation purification is carried out twice to obtain a refined extraction product of exopolysaccharide, and the refined extraction product of exopolysaccharide is mixed with agar and starch toprepare the heavy metal adsorbent. The heavy metal adsorbent has high adsorption efficiency, can be used directly in drinking water, and is safe and harmless.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a method for preparing a heavy metal adsorbent by using marine Pyrobacterium strains Background technique [0002] Most of the water used by people in production and life is tap water, which is mainly obtained by treating water drawn from groundwater or river water by water plants. Because some water sources are affected by industrial or domestic sewage discharges, the heavy metal content exceeds the standard. Although it is treated by a water plant before being used for human and livestock consumption, the content of some heavy metals is still at a high level, and some even exceed the standard; in addition, urban tap water pipelines Old age will also cause heavy metals such as lead to infiltrate into the water body and cause heavy metal content to exceed the standard; although there are water purifiers with heavy metal removal function on the market, the water purifier m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30C02F1/28C12P19/04C12R1/01C02F101/20
Inventor 穆军穆思源崔霞
Owner ZHEJIANG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com