Ruditapes philippinarum species real-time fluorescent PCR (polymerase chain reaction) specific detection system and application thereof

Abstract

The invention belongs to the technical field of biology, and particularly relates to a Ruditapes philippinarum species real-time fluorescent PCR (polymerase chain reaction) specific detection system and application thereof. The sequences of the primers and probe are as follows: 5'-ggttgaacagtctaccctcc-3' (forward); 5'-gctaacatactactacgcaaaa-3' (reverse); and 5'-tctcttcacgtaggtggtgtctcttcaatt-3' (probe). After the Ruditapes philippinarum species specific detection primers and probe are adopted to perform real-time fluorescent PCR amplification on CoxI gene of Ruditapes philippinarum, a specific amplification curve can be generated, thereby performing specific identification on the Ruditapes philippinarum components. The specific primers and probe are reasonably designed, and have the advantages of favorable specificity and high detection sensitivity when being used for Ruditapes philippinarum species detection. The fluorescent PCR analysis can be performed to accurately identify the Ruditapes philippinarum components, thereby having favorable specificity.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a real-time fluorescent PCR specific detection system and application of clam species. Background technique [0002] Variegated clam is a shellfish product belonging to the clam family. Its scientific name is Philippine clam. It is commonly known as clam in the south, clam in Liaoning, and clam in Shandong. Variegated clam is widely distributed in the north and south sea areas of my country. It grows rapidly, has a short breeding cycle, strong adaptability (wide temperature, wide salt, wide distribution), and long survival time out of water. It is an excellent shellfish suitable for artificial high-density breeding. It is one of the main marine economic shellfish in my country. Judging by morphological characteristics is the traditional method of identifying variegated clams, but these morphological characteristics are highly plastic, are greatly affected by the environme...

AI Technical Summary

Problems solved by technology

Judging by morphological characteristics is the traditional method of identifying variegated clams, but these morphological characteristics are highly plastic, are greatly affected by the environment, have artificial subjective tendencies, and there are abundant related species, and the morphological differences between related species Subtle, so the traditional morphological feature recognition method has the problems of recognition difficulty and recognition error

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