Environmental DNA macro bar code method for researching macrobenthic animal community structure

Abstract

The invention discloses an environmental DNA macro bar code method for researching a macrobenthic animal community structure. One technical scheme to be protected by the invention is the application of the primer pair in detecting the community structure of the macrobenthos in the water area to be detected. The primer pair is named as COI and is composed of a primer 1 and a primer 2, and the primer 1 and the primer 2 can be a sequence 1 and a sequence 2 in a sequence table. Compared with a traditional method, the COI primer provided by the invention is used for amplifying an environmental DNAsample of a to-be-detected water area, and sequencing and annotation analysis are carried out to obtain more obvious species, especially benthonic animal groups which are easy to escape, small in sizeand partial in life history in water; and the method can supplement benthonic animal abundance data which are easy to ignore, difficult to find and small in size in a traditional investigation method, effectively improves the identification level, species detection rate and abundance evaluation accuracy of the large benthonic animals, and is an effective and reliable new method for investigatingthe community structure of the large benthonic animals.

Description

technical field [0001] The invention relates to the field of molecular ecology, in particular to an environmental DNA macro-barcode method for studying macrobenthos community structure. Background technique [0002] Environmental DNA (eDNA) metabarcoding refers to extracting biological DNA fragments directly from water, soil, sediment and other environments, amplifying eDNA by designing universal primers, using high-throughput sequencing to obtain a large number of DNA sequences, and comparing them with reliable species. The DNA barcode reference sequence of taxonomic information is compared to determine the composition and abundance of target organisms in the environment, which is a new type of aquatic biological community structure and diversity detection technology. Compared with traditional aquatic biome structure survey techniques, this technique has the characteristics of high accuracy, friendliness to resources and the environment, easy detection of rare species, and ...

AI Technical Summary

Problems solved by technology

The traditional survey of macrobenthic community structure relies on dredgers to collect sediments to select macrobenthos from sediments. This survey method is time-consuming, laborious, and generally has problems such as incomplete investigation, difficult identification, and inaccurate estimation of abundance.

However, the environmental DNA method has not yet formed a mature and systematic standardized experimental process in the detection of macrobenthic community structure. The selection of general primers, the screening of sampling sample types, and the evaluation of the validity and reliability of annotation results have not yet been scientifically verified and tested. Evaluate

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