Process for testing bacteria gene chip in sewage

Abstract

This invention relates a gene chip detection method of bacteria in sewage. The procedures includes that selection of objective bacteria, the characteristic gene selected from different objective bacteria are used as target gene of gene detecting, PCR prime and species detecting probe are designed according to special gene of different bacteria, the gene chip is prepared according to the design. Then DNA of the bacteria is distilled, PCR amplifying reaction is done, then reactive product of the amplifying reaction is hybridized and product clearing. In this invention, the characteristic gene is used as target gene, and PCR prime and species detection probe are designed according to the characteristic gene of different bacteria, the prime designed has good specificity and good amplifying effect to bacteria, and without non-specificity amplifying. The distill method of pure bacteria is simple, and it can be done in 2 hours and gain ratio is high. The PCR amplification and crossover method are easy to realize, and the crossover detecting result is stable, so it is propitious to practical application of this technique.

Description

technical field [0001] The invention relates to a method for detecting a gene chip of bacteria, in particular to a method for detecting a gene chip of bacteria in sewage. Background technique [0002] In the prior art, the detection of bacteria in sewage mainly utilizes traditional microbiological methods, biochemical methods, serological methods and PCR methods (Santiago F.Gonzalez, Melissa J.Krug, MichaelE.NIelsen, et al.2004; Call , D., M. Borucki, and T. Besser. 200). These methods have some limitations: (1) due to the need for cultivation, it takes a long time; (2) cannot distinguish bacteria with close relatives; (3) cannot identify unknown bacteria; (4) cross-reaction and other false positives; (5) ) sensitivity issues (false negatives). The use of gene chip technology to quickly detect the pollution and harm of bacteria to the environment, human body, animals and plants can efficiently detect the pollution caused by bacteria, and can also help researchers find and ...

AI Technical Summary

Problems solved by technology

At present, in most gene chip studies, bacterial universal primers are designed in the constant region of 16S rRNA, and detection probes are designed in the variable region. (Salmonella, Mycogella, Escherichia coli, etc.) The 16SrRNA sequence of the probe is basically the same, so it can only be detected as a type of bacteria

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