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Water environment DNA intelligent collecting device and collecting method

A technology of intelligent collection and water body, applied in the field of environmental DNA detection, can solve the problems of low efficiency of biological sample sampling process, easy changes of biological samples, large calculation error of water filtration amount, etc. big effect

Pending Publication Date: 2019-03-01
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the low efficiency of the sampling process of biological samples in the existing water body environment, the large error in the calculation of the filtered water volume, the easy change of biological samples after collection, and the problems that the existing sampling equipment is easy to be blocked due to pipeline damage, the present invention provides a water body environment DNA intelligent collection device and collection method, the device can be used for portable quantitative enrichment and sampling, and can also be used with carrier equipment such as unmanned ships or drones, which can greatly reduce labor intensity and improve sample accuracy and representativeness

Method used

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  • Water environment DNA intelligent collecting device and collecting method
  • Water environment DNA intelligent collecting device and collecting method

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Effect test

Embodiment 1

[0061] like figure 1 As shown in the figure, an intelligent DNA collection device for water environment includes a box body 1, an intelligent control mechanism located inside the box body 1, and a sampling main body mechanism; the sampling main body mechanism includes a sample collection unit, a pressure relief unit, a water emptying unit unit and three-way valve 20;

[0062] In this embodiment, the intelligent control mechanism includes a main control board 10 and a GPS positioning block 12 connected to the main control board 10 , a power supply system 15 connected to the main control board 10 , a display screen 16 and a hand control board 11 , and a wireless control board 11 . The remote controller 17 wirelessly connected to the board 13 ; the main control board 10 is used to control the opening or closing of the collection unit and the water emptying unit, and to receive the positioning information of the GPS positioning block 12 . The manual control panel 11 is used to pr...

Embodiment 2

[0072] The concrete steps of a kind of water environment DNA intelligent sampling method in the present embodiment are as follows:

[0073] A. build a kind of water environment DNA collection device as claimed in claim 1~8;

[0074] B. Parameter preset: use the manual control board 11 to preset the sampling parameters when the device is running on the main control board 10;

[0075] C. Sample collection: After the collection device is placed at the collection site, the system is started. The main control board 10 issues commands according to the command parameters to control the sampling unit to work. The solenoid valve II3 and the water pump 18 are turned on. Enter the collector 4 for sample sampling; at this time, the water emptying unit and the pressure relief unit are in a non-working state; the water biological samples in the water sample are enriched into the enrichment collector; the excess water flows through the flow meter 5 and passes through the single The device i...

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Abstract

The invention discloses a water environment DNA intelligent collecting device and collecting method, and belongs to the technical field of environment DNA detection. The water environment DNA intelligent collecting device and collecting method integrate the functions of intelligent control, sample collection, residual liquid emptying, pipeline blockage and pressure relief protection, can collect not only water biological samples but also water samples, can effectively improve the efficiency of the biological sample sampling process and water filtering amount calculation accuracy, ensure the sample timeliness and make the water sample detecting environment closer to the natural environment, thereby having the advantages of being not easy to block, simple in structure, reasonable in design and easy to manufacture.

Description

technical field [0001] The invention belongs to the technical field of environmental DNA detection, and more particularly, relates to an intelligent collection device and collection method of water body environmental DNA. Background technique [0002] Species distribution and species richness are the main issues in the study of species diversity in freshwater ecosystems, which play a crucial role in implementing biodiversity conservation measures. Obtaining DNA from species living environment to reflect species distribution, abundance and other information is a non-invasive and rapid species detection method. With the rapid development of high-throughput sequencing technology, species diversity research methods based on environmental DNA and molecular biology technology (eDNA metabarcoding technology) have begun to be more and more applied to water ecological health assessment, which has significantly improved our understanding of The level of understanding of species diver...

Claims

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Application Information

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IPC IPC(8): G01N1/14C12M1/26
CPCG01N1/14C12M33/14G01N2001/1418
Inventor 杨江华张效伟付召军
Owner NANJING UNIV
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