Filter membrane assembly and collector for DNA enrichment in water environment and application method

A water environment and collector technology, applied in the field of environmental DNA detection, can solve the problems of effective filtration area loss, slow filtration speed, low filtration flux, etc., and achieve the effect of improving filtration efficiency, preventing clogging, and ensuring filtration flux

Active Publication Date: 2020-09-11
南京易基诺环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of existing water environment DNA enrichment filter membranes, which are prone to clogging, slow filtration speed and low flux during the process of filtration and enrichment, the present invention maintains a certain Gaps can avoid the loss of effective filtration area and blockage of filter holes caused by the stacking of filter membranes; thus effectively solving the problem of low filtration flux and blockage

Method used

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  • Filter membrane assembly and collector for DNA enrichment in water environment and application method
  • Filter membrane assembly and collector for DNA enrichment in water environment and application method
  • Filter membrane assembly and collector for DNA enrichment in water environment and application method

Examples

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Embodiment 1

[0062] Such as figure 1 , 2 As shown, the filter membrane assembly 440 provided in this embodiment for the enrichment of DNA in the water environment is a "3D" laminated filter membrane, and the filter membrane assembly 440 includes three layers of filter membranes 100 that are sequentially stacked from top to bottom, The edges (approximately 3mm) between the layers of filter membranes are bonded by pressure-sensitive adhesives to form filter membrane bonding edges 300; The filter pore diameter of filter membrane 100, the filter pore diameter of upper filter membrane 110 described in the present embodiment is 20 μ m; The filter pore diameter of described middle filter membrane 120 is 5 μ m; The filter pore diameter of described lower layer filter membrane 130 is 0.2 μm.

[0063] Between the upper filter membrane 110 and the middle filter membrane 120, between the middle filter membrane 120 and the lower filter membrane 130 are filled with microspheres 200 of silicon dioxide ...

Embodiment 2

[0072] Such as figure 1 , 2As shown, the filter membrane assembly 440 provided in this embodiment for the enrichment of DNA in the water environment is a "3D" laminated filter membrane, and the filter membrane assembly 440 includes three layers of filter membranes 100 that are sequentially stacked from top to bottom, The edges (about 3 mm) between the layers of filter membranes are bonded by a pressure-sensitive adhesive to form a filter membrane bonding edge 300; The aperture of the filter hole of the membrane 100, the filter aperture of the upper filter membrane 110 described in the present embodiment is 100 μm; the filter aperture of the middle filter membrane 120 is 20 μm; the filter aperture of the lower filter membrane 130 is 1 μm .

[0073] Between the upper filter membrane 110 and the middle filter membrane 120, between the middle filter membrane 120 and the lower filter membrane 130 are filled with zirconia microspheres 200 with a particle diameter of 2mm, between t...

Embodiment 3

[0082] Such as figure 1 , 2 As shown, the filter membrane assembly 440 provided in this embodiment for DNA enrichment in the water environment is a "3D" laminated filter membrane, including three layers of filter membranes 100 stacked sequentially from top to bottom, and each layer of filter membranes 100 The edges (about 3 mm) between them are bonded by pressure-sensitive adhesive to form the filter membrane adhesive edge 300; and according to the order from top to bottom, the filter pore diameter of the filter membrane 100 in the upper layer is larger than that of the filter membrane 100 in the lower layer aperture.

[0083] The upper filter membrane 110 described in this embodiment is a nylon filter membrane with a filter pore diameter of 100 μm; the middle filter membrane 120 is a nylon filter membrane with a filter pore diameter of 20 μm; the lower filter membrane 130 is a filter pore diameter of 0.45 μm mixed cellulose filters.

[0084] Between the upper filter membra...

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Abstract

The invention discloses a filter membrane assembly and collector for DNA enrichment in water environment and an application method, and belongs to the technical field of environmental DNA detection. The filter membrane assembly includes at least two layers of filter membranes stacked in sequence from top to bottom, the filter pore size of each layer of the filter membrane is arbitrarily selectively reduced linearly or stepwise; and a certain gap is maintained between two upper-lower adjacent layers of filter membranes, and filter holes between the two adjacent layers of the filter membranes are arranged in a staggered manner. By using the filter membrane assembly for DNA enrichment in the water environment, gradient filtration of biological samples in water samples can be achieved, clogging of the filter membranes is avoided, the filtration capacity of the filter membranes is increased, the filtration flux and filtration efficiency of the water samples are increased, and the subsequentDNA extraction efficiency can further be improved.

Description

technical field [0001] The invention belongs to the technical field of environmental DNA detection, and more specifically relates to a filter membrane assembly, a collector and an application method for DNA enrichment in a water environment. Background technique [0002] Species distribution and species richness are the main issues in the study of species diversity in freshwater ecosystems, and play a vital role in the implementation of biodiversity conservation measures. Obtaining DNA from the living environment of species to reflect species distribution, abundance and other information is a non-invasive and rapid means of species detection. With the rapid development of high-throughput sequencing technology, species diversity research methods based on environmental DNA and molecular biology technology (eDNA metabarcoding technology) have been increasingly applied to the evaluation of water ecological health, which has significantly improved Our level of awareness of speci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10B01D63/08C02F1/44
CPCC12N15/1006B01D63/082C02F1/44Y02A90/40
Inventor 杨江华张效伟
Owner 南京易基诺环保科技有限公司
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