Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for fast extracting AM epiphyte environment DNA in plant rhizosphere soil

A technology of plant roots and fungi, applied in the fields of molecular biology and applied microbiology, can solve the problems of undiscovered public reports, achieve the effect of reducing degradation and promoting the release of DNA

Inactive Publication Date: 2008-03-26
YUNNAN UNIV
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Through document search, do not find the public report of the same document as the content of the present invention

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Extraction of AMF genomic DNA from the rhizosphere soil of Dodonaea viscose:

[0040] Take by weighing 100g of willow plant rhizosphere soil, collect AM fungus-related structures (AMF spores, extraroot hyphae, etc.) DNA was extracted with 20% Chelex-100 resin, and nested-PCR was performed, and the AMF target gene was obtained by cloning and sequencing. And submit the obtained gene to GenBank, the sequence accession number is EU118739.

Embodiment 2

[0042] Genomic DNA extraction of AM fungi in rhizosphere soil of Heteropogon contortus;

[0043] It is basically the same as in Example 1, and the obtained gene is submitted to GenBank, and the sequence accession number is EU118750.

Embodiment 3

[0045] Genomic DNA Extraction of AM Fungus from D. viscose Root:

[0046] Weigh 2 g of fresh willow root system, sterilize the surface with 75% ethanol, cool and grind into powder with liquid nitrogen, add 1000 μl CTAB solution and 20% Chelex-100 resin for DNA extraction according to the method of the present invention, and perform nested-PCR , the objective gene of AM fungus was obtained by cloning and sequencing. And submit the obtained gene to GenBank, the sequence accession number is EU118692.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to process of fast extracting AM fungal genome DNA in plant rhizosphere soil environment, and belongs to the field of molecular biology and applied microbiology. The process includes the first wet screening to eliminate great amount of PCR proliferation limiting factors from rhizosphere soil, collecting AM fungal related structures of rhizosphere soil for DNA extraction, mechanically breaking wall and adding CTAB to promote DNA release, and further purifying with Chelex-100 resin. The process is easy, simple and fast, and has great DNA extracting amount, capacity of being concentrated and purified and other advantages. The present invention has excellent application foreground.

Description

Technical field: [0001] The invention relates to a method for quickly extracting environmental DNA of AM fungi in plant rhizosphere soil, belonging to the fields of molecular biology and applied microbiology. Background technique: [0002] Arbuscular mycorrhizal fungus (Arbuscular mycorrhizal fungus, AMF) is a class of soil microorganisms widely distributed in nature, it can form a mutualistic symbiont with the roots of more than 90% of terrestrial vascular plants - arbuscular mycorrhizal fungi Mycorrhiza, AM). More and more studies have shown that a large number of AMF extrarhizomycelia in the rhizosphere play important ecological functions in the soil ecosystem. It is an important factor in the composition of flora, and it is also an important factor affecting the allocation of resources in the ecosystem, the stability of the ecosystem, species diversity and productivity. Arbuscular mycorrhizae appear to affect the processes and functions of living systems at different o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C12N15/10
Inventor 李涛赵之伟
Owner YUNNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com