Method for monitoring and evaluating fish passage effects based on environmental DNA

Abstract

The invention discloses a method for monitoring and evaluating fish passage effects based on environmental DNA. The method comprises the following steps: S1, setting three sampling points at an entrance, exit and rest pool of a fish passage, and collecting water samples and performing storage; S2, performing suction filtration on the collected water samples, and then performing extraction of totalenvironmental DNA; S3, using the extracted total environmental DNA as a template, and using designed primers to PCR amplify a 16S rDNA sequence to obtain a PCR product; S4, recovering and purifying the PCR product, and performing high-throughput sequencing to obtain a PCR product sequence; S5, establishing a fish 16S rDNA sequence alignment database; S6, searching for homologous sequences in theNCBI database and the established database through BLAST, and determining fish species information; and S7, counting the obtained fish species information, and completing the monitoring and evaluationof the fish passage effects. Compared with other existing methods, the method does not need to carry out a large number of fishing operations or instrument operations, is simple in sampling, cannot cause harms to fish, and thereby is conducive to the protection of fish.

Description

technical field [0001] The invention relates to the technical field of molecular ecology, in particular to an environmental DNA-based method for monitoring and evaluating the effect of fish passages. Background technique [0002] Fish passage is an artificial facility for fish to pass through sluices or dams. The construction of water conservancy projects destroys the connectivity of the river, hinders the migration of fish and the transfer of materials in the river, and seriously threatens the biodiversity of fish. , in order to alleviate the negative impact caused by water conservancy projects, the natural living habits of fish are generally protected by building fish passages on sluices or dams, and fish migration and population exchanges between upstream and downstream are realized; [0003] The research on the monitoring and evaluation of the effect of fish passages started late, and there is no uniform standard for the monitoring and evaluation of the effects; although...

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Problems solved by technology

[0005] The present invention provides a method for monitoring and evaluating the effect of fish passages based on environmental DNA, which can effectively solve the problem that the research on monitoring and evaluating the effects of fish passages proposed in the above-mentioned background technology started relatively late, and there is no unified standard for the monitoring and evaluation of effects; Although relevant studies have been reported, most of the tens of thousands of fish passages around the world have not been monitored and evaluated. The methods usually used to monitor and evaluate the effects of fish passages include stretching nets, blocking methods, video monitoring, Hydroacoustics and remote sensing methods, etc., but these methods also have disadvantages, such as damage to fish, long time consumption, high cost of equipment, and inconvenient operation

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