Method for evaluating integrity of benthonic animals based on environmental DNA macro bar code technology

A benthic animal and macro-barcode technology, applied in biochemical equipment and methods, general water supply saving, microbial measurement/inspection, etc., can solve the problems of limited number of identified species, lack of representativeness, low monitoring efficiency, etc., and achieve high Classification and identification ability, less material resources and time, and the effect of improving accuracy

Pending Publication Date: 2021-09-17
NANJING UNIV
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Problems solved by technology

[0006] In view of the low monitoring efficiency, time-consuming and labor-intensive construction methods of the benthos integrity index, which rely heavily on the professional knowledge and experience of the appraiser, and the number of identified species is limited, it cannot reflect the changes in the genetic composition of benthic animals under environmental gradient changes. There are few candidate parameters for constructing B-IBI index, and the problem of lack of representativeness, the present invention provides a kind of benthic animal biological integrity based on environmental DNA (Environmental DNA, eDNA) macro barcode (Metabarcoding) monitoring method construction Evaluation method for more efficient, economical and high-resolution assessment of water ecological health

Method used

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  • Method for evaluating integrity of benthonic animals based on environmental DNA macro bar code technology
  • Method for evaluating integrity of benthonic animals based on environmental DNA macro bar code technology
  • Method for evaluating integrity of benthonic animals based on environmental DNA macro bar code technology

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Experimental program
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Embodiment 1

[0060] Embodiment of the present embodiment from the sample acquisition lakes and reservoirs 30 sites Jiangsu Taihu basin, following sub-steps of a method embodiment will be specifically described.

[0061] (1) a reference point and the damaged point determination

[0062] Water binding region ecological characteristics and environmental management objectives, according to the water quality is relatively good, relatively light eutrophication, aquatic vegetation high coverage, a relatively low intensity jamming determination conditions, the selection conditions in accordance with the reference state, the final choice pond horse Reservoir, Sha Zhu, Pu village, mountain pepper, Dasi reservoir south, Man Shan, a mountain spring stuff Taihu Lake Basin lakes and reservoirs (lakes and reservoirs) reference point benthic fauna integrity index construction, and the remaining points are considered by loss point.

[0063] (2) Sample collection and DNA extraction environment

[0064] By Peter...

Embodiment 2

[0094] Samples collected from 37 sites in rivers and streams Taihu Lake Basin. To give 37 rivers and streams through the same site as in Example 1, step Zoobenthos integrity analysis results as follows:

[0095] (1) The selection of the reference state condition, the final choice For fishing bridge, Rujiangshuidao _ Dai Louheng male, jiuxian, air traffic control station, Taipu gate, bay off the bottle, as benthic animals Zhaotun perfection index Taihu Basin Construction of a reference point, the remaining non-reference points are damaged point.

[0096] (2) The distribution filter, all indices wide variable range of the spatial change in response to stress is large, the integrity of the indicator system may be involved in building, so that all entries are indices into the box 27 discriminant analysis.

[0097] (3) the housing discriminant analysis showed that a total of 13 candidate index IQ≥2, some parameters of the distribution box, see Figure 4 .

[0098] (4) biological indicat...

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Abstract

The invention discloses a method for evaluating biological integrity of benthonic animals of a freshwater ecosystem based on environmental DNA macro bar code monitoring data, and belongs to the technical field of biology. Based on the results of monitoring of the benthonic animals through environmental DNA macro bar codes, the method comprises a series of monitoring and evaluation processes including (1) determining a reference point and a damaged point; (2) collecting an environmental DNA sample; (3) performing biological monitoring on an eDNA macro bar code; (4) defining and calculating candidate biological indexes based on the eDNA macro bar code monitoring; (5) screening the candidate indexes; (6) selecting the biological indexes to calculate the biological integrity score of the benthonic animals; and (7) dividing health levels and the like. The method provides complete and easy-to-operate guidance for the evaluation of the biological integrity of the benthonic animals of the freshwater, and can be widely applied to the evaluation of the integrity of the benthonic animals and the evaluation of the water ecological health condition of freshwater ecosystems such as streams, rivers, lakes and reservoirs.

Description

Technical field [0001] The present invention belongs to the field of assessment and control environment and, more particularly, to a method of evaluation of the integrity of the environmental DNA Zoobenthos macro based bar code technology. Background technique [0002] Environmental DNA (eDNA) refers to a mixture of genomic DNA of different organisms in environmental samples all were found, including water sample DNA, sediment DNA, biogenic DNA; biogenic DNA refers to biological tissue extracted DNA, e.g. skin, hair and so on. DNA barcode (Barcode DNA) refers to a biological body to represent the species, standard, there is enough variation, and relatively easy to amplify short DNA fragments, it has become an important tool for the study of ecology. [0003] DNA macro barcodes (Metabarcoding) technology is a tradition after DNA barcoding and high-throughput sequencing technology derived from the combination of research methods capable of noninvasively investigate species richness...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686G06Q10/06G06Q50/26
CPCC12Q1/6888C12Q1/686G06Q10/06393G06Q50/26C12Q2600/156C12Q2563/185Y02A20/152
Inventor 张效伟张丽娟杨江华
Owner NANJING UNIV
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