The invention discloses a method for preparing animal probiotics fodder by fermenting edible mushroom waste, straw and pasture. According to the method, the edible mushroom waste, the straw and the pasture serve as ingredients, the three types of animal probiotics, namely aerobiotic probiotic fungi, aerobiotic probiotic bacteria and anaerobic probiotic bacteria, serve as fermentative strain for conducting multi-step fermentation, the enrichment technique, the liquid fermentation technology and the solid fermentation technique are adopted in a combined mode for completing the fermentation and preparation of the animal probiotics fodder through seven steps, in the fermentation process, the growth and toxin metabolism of pathogenic bacteria are restrained effectively, the nutritional properties and palatability of the ingredients are improved, and finally the fodder containing high-concentration animal probiotics flora is obtained. According to the technical method, the fodder can be used by conducting fermentation for 4-5 days, 40-50 days are required by a traditional ensilage fermentation method, the method has the advantages of being short in elapsed time, little in manpower cost, high in efficiency, easy to learn and master, easy to popularize widely and the like, and the defects of the traditional fermentation method are overcome thoroughly.