Intelligent collection device for environment DNA in marine water body

Abstract

The invention provides an intelligent collection device for environmental DNA in a marine water body, and relates to the technical field of DNA collection devices. The intelligent collection device for environment DNA in marine water comprises a remote control ship, the remote control ship is provided with a pull rope and a winding and unwinding assembly used for winding and unwinding the pull rope, one end of the pull rope is connected with the winding and unwinding assembly, and the other end of the pull rope is provided with a sampling barrel; a water storage cavity is formed in the sampling barrel, a first opening communicated with the water storage cavity is formed in the top of the sampling barrel, and a first electromagnetic valve is arranged at the first opening; a second opening communicated with the water storage cavity is formed in the bottom of the sampling barrel, and a second electromagnetic valve is arranged at the second opening; and the intelligent collection device further comprises a processor, a power source and a remote control module, the remote control module is wirelessly connected with the processor, and the retractable assembly, the power source, the first electromagnetic valve and the second electromagnetic valve are all electrically connected with the processor. By the adoption of the collecting device, marine water samples can be conveniently collected, operation is convenient and fast, and manpower is saved.

Description

technical field [0001] The invention relates to the technical field of DNA collection devices, in particular to an intelligent collection device for environmental DNA in marine water bodies. Background technique [0002] Species distribution and species richness are the main issues in the study of ecosystem species diversity, which play a vital role in implementing biodiversity conservation measures. Obtaining DNA from species living environment to reflect species distribution, abundance and other information is a non-invasive and rapid means of species detection. With the rapid development of high-throughput sequencing technology, more and more species diversity research methods based on environmental DNA and molecular biology technology (eDNA metabarcoding technology) have been applied to the evaluation of water ecological health, which has significantly improved our understanding of Levels of awareness of ecosystem species diversity. The earliest application of environm...

AI Technical Summary

Problems solved by technology

However, this method has the following obvious defects: (1) manual operation, low efficiency: restricted by the coast of the water body, ships, etc., the sampling range is limited, and the sample representativeness is poor; and it costs too much manpower and material resources, and the work efficiency is low; (2) It is difficult to accurately control the sampling position: Affected by the current, the plankton net is easy to drift away from the sampling point when sampling, and ships and many other factors make it impossible to sample ships in some waters; (3) The calculation error of the filtered water is large: due to Due to changes in the sampling depth of the plankton net, there is a large error in the estimated water filtration volume based on the release length of the steel wire rope, sampling angle, and sampling time, and the phytoplankton biodensity calculated based on the water filtration volume is inconsistent with the actual situation; (4) Water environment Samples are very easy to change. During the period from collection to analysis and determination, due to changes in environmental conditions, the metabolic activities, chemical and physical effects of organisms in the water will cause changes in the collected samples and cause environmental DNA. The change or even degradation will affect the detection rate of species, and at the same time, it will also affect the detection results such as species distribution

And these changes are very fast, therefore, necessary protective measures must be taken to preserve the samples

Although eDNA metabarcoding technology has greatly improved the efficiency and accuracy of environmental monitoring as an emerging environmental monitoring method, the traditional biological sampling method does not meet the needs of this new monitoring technology, and also limits the application of this method in environmental protection systems. Large-scale promotion and application

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