The invention belongs to the field of metal material structure analysis sample preparation and structure observation characterization, and particularly relates to an in-situ EBSD observation method for microcosmic orientation evolution of recrystallized grains of magnesium alloy. The method comprises the steps of firstly, carrying out mechanical polishing on a magnesium alloy sample, then preparing indentation mark points on the surface to be detected, and conducting EBSD observation for the first time on a target area after ion etching; and taking out the sample, carrying out recrystallization annealing heat treatment and ion etching, finding the same area according to the indentation mark points, carrying out EBSD observation for the second time, and similarly, completing subsequent required in-situ EBSD observation. According to the method, in-situ tracking characterization of recrystallization nucleation, grain growth and microscopic orientation evolution in the same area in the recrystallization annealing process of the magnesium alloy is achieved, and the problems that the magnesium alloy is low in calibration rate due to a surface stress layer, an oxide layer and pollutants,accurate positioning cannot be achieved, and in-situ EBSD observation is difficult to achieve are solved. The process is simple and controllable, the technical requirements on sample preparation of experimenters are low, and the experiment success rate is high.