Method for preparing protein-based surfactant by opening protein disulfide bond

A surfactant-based, surface-active technology, used in chemical instruments and methods, chemical/physical processes, dissolution, etc., can solve the problem of destroying the structural integrity of proteins, the natural properties of proteins, and the destruction of protein polar and non-polar groups, etc. problems, to achieve the effect of improving surface active properties, wide application range and broad practical prospects

Inactive Publication Date: 2009-12-02
GUANGXI UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method destroys protein polar and non-polar groups, destroying the integrity of protein structure and the natural properties of proteins

Method used

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  • Method for preparing protein-based surfactant by opening protein disulfide bond
  • Method for preparing protein-based surfactant by opening protein disulfide bond
  • Method for preparing protein-based surfactant by opening protein disulfide bond

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing protein-based surfactants by opening protein disulfide bonds using peroxides in different amounts, comprising the following specific steps:

[0026] A. Preparation of water-soluble protein solution and determination of its disulfide bond content:

[0027] Put 50g of soybean protein isolate into a centrifuge tube, add 500mL of distilled water, put it in a shaker, control the temperature at 20±2°C, shaker speed: 50 times / min, centrifuge after 60min, and the centrifugal speed is 4000 rpm , then pour the supernatant into a 2500mL volumetric flask, then add 500mL of distilled water, put it in a shaker, centrifuge after 60min, repeat this three times, then fix the water-soluble protein solution to 2500mL, and then use Kjeldahl to determine nitrogen The content of water-soluble protein analyzed by the method was 0.01246g / mL, and the content of disulfide bonds before opening was measured by spectrophotometer: 0.3034μmol / mL (see attached table 1);

[0028...

Embodiment 2

[0043]A method for preparing protein-based surfactants by opening protein disulfide bonds. A of the method, preparing a water-soluble protein solution and measuring the content of its disulfide bond, B, opening the protein disulfide bond: 1. preparing peroxyformic acid solution in the method: the process is the same as in Example 1, the difference is: use In the process of peroxide oxidation of protein, the reaction time of peroxide is different, and the specific experimental steps are as follows:

[0044] ②Use peroxide to oxidize protein: Add 4 mL of peroxyformic acid solution with a concentration of 1 mol / L to 6 flasks containing 200 mL of water-soluble soybean protein solution with a protein content of 0.01246 g / mL, and mix the mixture Place it in an ice-salt bath with a temperature of about minus 10°C, and react in the dark. The reaction time of the solution in each flask is set as: 1h, 2h, 3h, 4h, 5h, 6h, and then add 2mL of ethanol to make it Terminate the reaction, obt...

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Abstract

The invention relates to a method for preparing protein-based surfactant by opening protein disulfide linkage. The method is characterized by converting disulfide linkage into sulfonic group by oxidizing disulfide bond in protein of peroxide, and opening the folded coiling structure of the protein to represent the surface activity properties so as to prepare the protein-based surfactant which has the functions of emulsification, foaming, defoaming, washing, decontamination, soakage, and the like; by introducing polar group or nonpolar group into a protein side chain with opened disulfide bond, the surface activity properties of peptide fragments can be further improved or increased; moreover, by adopting treatments including acetylization, succinylation, acylation or phosphorylation, protein surface activity properties such as surface tension, emulsification, foaming, defoaming, washing, decontamination and soakage can be changed further. The method furthest prevents protein polar group and nonpolar group from being damaged, and maintains the structural integrity and property naturalness of protein molecules, thereby converting the protein into natural high-performance surfactant.

Description

Technical field: [0001] The invention relates to a method for preparing protein-based surfactants, in particular to a method for preparing protein-based surfactants by opening protein disulfide bonds. Background technique: [0002] Protein is one of the most important biological macromolecules, which exists in all organisms. It is the main component of cells, the basic material of life, and a pure natural nutrient. According to the polarity of side chain groups, more than 20 kinds of amino acids that make up proteins can basically be divided into two categories: polar amino acids and non-polar amino acids. Therefore, all proteins contain both polar and non-polar groups. An ideal amphiphilic molecule or surface active molecule should have ideal surface active properties in theory. However, due to the irregular or specific distribution of polar and non-polar amino acids in each protein peptide chain, as well as the influence of protein spatial structure and spatial structural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F17/30C09K23/30
Inventor 李军生李丽娜程海涛阎柳娟
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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