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Immune-electrochemical sensor based on AuNPs@AgNCs nano composite material, construction and applications thereof

A nanocomposite material, electrochemical technology, applied in the field of electrochemical immunoassay, to achieve the effect of uniform size and good stability

Inactive Publication Date: 2015-01-07
CENT SOUTH UNIV
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Problems solved by technology

However, this signal amplification method is limited by the fact that there is only one silver nanocluster on a probe. If many silver nanoclusters on a nanoprobe can be obtained, it can maintain its inherent catalytic reduction of H 2 o 2 The peroxidase-like activity can also improve the amplification efficiency, which will greatly improve the application effect of the probe

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  • Immune-electrochemical sensor based on AuNPs@AgNCs nano composite material, construction and applications thereof
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  • Immune-electrochemical sensor based on AuNPs@AgNCs nano composite material, construction and applications thereof

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Embodiment Construction

[0047] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0048] The construction of the immunoelectrochemical sensor of the present invention and the immunoassay are schematically shown as figure 1 :

[0049] (1) Preparation of DNA-AgNCs: 50 μL 100 μM template strand S2: 3’-ACG CAT GCC GGC CCC TAA CTC CCC-5’ and 0.8 μL 50 mM AgNO 3 The solution was added to 50 μL of 20 mM phosphate buffer (PBS, pH=7.0), so that the Ag + : S2=8. Then, 0.8 μL of 50 mM freshly prepared NaBH 4 The solution was added to the above solution at 0°C, the resulting solution was shaken vigorously for 1 min, and the S2 / AgNCs were placed in a refrigerator at 4°C overnight in the dark.

[0050] (2) Preparation of AuNPsAgNCs nanocomposites: 25 μL 40 μM DNA single-stranded S1: 3'-SH-TTT TT GCC GGC ATG CGT-5' and 25 μL streptavidin-labeled gold nanoparticles (SA-AuNPs, sigma (purchased) mix, shake for 1 hour, let stand for 24 hours...

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Abstract

The invention discloses an immune-electrochemical sensor based on an AuNPs@AgNCs nano composite material, construction and applications thereof. Ab1 is fixed by graphene, PSA is taken as the target analyte, SA-AuNPs@AgNCs labeled by streptavidin is easily combined with monoclonal antibody Ab2(biotin-Ab2) which has been modified by biotin, and thus a PSA immunity sensor can be prepared by a sandwich interlaying method through the specific reactions between an antibody and an antigen. When the immune sensor is used, silver and H2O2 are precipitated on the sensor through electric catalytic reduction reactions, the detection is rapid and sensitive, has good specificity, and is capable of detecting the concentration of PSA with an ultralow content. The concentrations of f-PSA and t-PSA in serum of prostatic hyperplasia patients and prostatic cancer patients can also be detected by the sensor, so the sensor has an important meaning for diagnosis and identification on gray areas in prostatic cancer diagnosis.

Description

technical field [0001] The invention belongs to the technical field of electrochemical immunoanalysis, and in particular relates to an immunoelectrochemical sensor based on AuNPsAgNCs nanocomposite material and its construction and application method. Background technique [0002] Immunoassay is a powerful analytical tool for the specific detection of cancer markers in clinical samples by combining high-affinity antigen-antibody. Electrochemical immunosensors have received special attention due to their ease of use, rapid response, and portability, and signal amplification methods have been widely used to develop ultrasensitive immunosensors for the detection of tumor markers. The multi-enzyme probe based on nanomaterials is the most popular method to amplify the detection signal, and peroxidase is the most common, but the enzyme is easily affected by temperature, humidity and environment, and is easy to inactivate, time-consuming, preparation and purification The process i...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N27/26G01N33/5438G01N33/57415G01N33/57434G01N2800/342G01N2800/365G01N2800/7028
Inventor 邓留韩雅静李理刘又年卢红梅
Owner CENT SOUTH UNIV
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