Ochratoxin A fluorescence detection test strip and application thereof

A technology for detection of ochratoxin and fluorescence, which is applied in the field of fluorescence detection, can solve the problems of relatively high requirements for detection materials and expensive equipment, and achieve the effect of convenient use, low cost, and fast economy

Inactive Publication Date: 2010-12-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the main methods for detecting OTA are thin-layer chromatography (TLC), enzyme-linked immunosorbent assay (ELISA), and high-performance liquid chromatography (HPLC). It is also relatively high, and requires complicated sample pretreatment to carry out

Method used

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  • Ochratoxin A fluorescence detection test strip and application thereof
  • Ochratoxin A fluorescence detection test strip and application thereof

Examples

Experimental program
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Embodiment Construction

[0023] Further description will be made below in conjunction with the accompanying drawings.

[0024] 1. Ochratoxin A fluorescence detection test strip, including: 1. Lower absorbent pad, 2. Quantum dot coupled Aptamer①, 3. Streptavidin-biotin-Aptamer②, 4. Streptavidin-biotin-Aptamer③, 5. Upper absorbent pad, 6. Nitrocellulose membrane, 7. Bottom plate.

[0025] Among them, streptavidin-biotin-Aptamer② was used as the detection band, and streptavidin-biotin-Aptamer③ was used as the reference band.

[0026] The width of the film strip is not less than 3.5mm, and the appearance is flat, the material is firmly attached, and the liquid migration speed is not less than 5mm / min.

[0027] 2. Preparation of ochratoxin A fluorescent test strips

[0028] (1) Biotin-modified aptamers Aptamer② and Aptamer③ were reacted with streptavidin at a molar ratio of 1:4 at room temperature for 1 hour to generate streptavidin-biotin-Aptamer② and streptavidin-biotin-Aptamer③.

[0029] (2) Quantum ...

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Abstract

The invention discloses an ochratoxin A fluorescence detection test strip and application thereof, relates to a method for detecting ochratoxin A by using a fluorescence test strip of a quantum dot-labeled aptamer, and belongs to the technical field of fluorescence detection. The test strip comprises a lower water-absorbent pad (1), a quantum dot-coupled Aptamer 1 (2), a streptavidin-biotin-Aptamer 2 (3), a streptavidin-biotin-Aptamer 3 (4), an upper water-absorbent pad (5), a nitrocellulose membrane (6) and a bottom plate (7). By using a chromatography one-step competition principle, the test strip semiquantitatively detects ochratoxin A residue quantity in a semiquantitative detection sample through upper and lower colorimetric belts thereof, rapidly and accurately detects whether the sample contains the ochratoxin A within 15 min to determine whether the ochratoxin A is overproof, can meet the requirement of food safety on the detection of the ochratoxin A residue quantity, and is suitable for feeds, meat producing plants and government detection mechanisms; and compared with the prior art, the test strip has the characteristics of convenient use, economy, rapidness, simple manufacturing and low cost.

Description

technical field [0001] The invention relates to an ochratoxin A fluorescence detection test strip and its application, and relates to a method for detecting ochratoxin A with a quantum dot-labeled aptamer fluorescent test strip, which belongs to the technical field of fluorescence detection. Background technique [0002] Ochratoxin A, English name: ochratoxinA (OTA) is a secondary metabolite produced by certain species of Aspergillus and Penicillium, which directly endangers human health. The toxin mostly occurs in humid areas below 30°C. In vivo and in vitro tests have shown that OTA is genotoxic and causes DNA damage. Rat and mouse tests can penetrate the placenta and cause mutagenic effects. For patients with upper urinary tract tumors, the level of OTA in blood is higher than that of other populations, so the tentative weekly tolerance intake (PTWI) is 100ng / kg. Considering that wheat, barley and rye are the main food species ingesting OTA, it was decided to formulate t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N21/78C12Q1/68
Inventor 胥传来马文蔚陈伟王利兵匡华刘丽强胡拥明彭池方
Owner JIANGNAN UNIV
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