Myocardial infarct early diagnosis liquid phase chip and method for producing the same

A liquid phase chip and early diagnosis technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity and specificity, and achieve the effects of simple and easy preparation method, high detection efficiency and high sensitivity

Inactive Publication Date: 2008-09-24
SUREXAM BIO TECH
View PDF0 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to provide an early diagnosis of myocardial infarction that detects multiple myocardial injury markers synchronously in view of the def

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Myocardial infarct early diagnosis liquid phase chip and method for producing the same
  • Myocardial infarct early diagnosis liquid phase chip and method for producing the same
  • Myocardial infarct early diagnosis liquid phase chip and method for producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Preparation of Liquid Chip for Early Diagnosis of Myocardial Infarction and Antigen Detection

[0061] The capture antibody of the present invention is a monoclonal antibody that can specifically bind to CK-MB, Mb, cTnI, GPBB and H-FABP; Monoclonal or polyclonal antibodies specifically binding to GPBB and H-FABP.

[0062] The capture antibodies and detection antibodies of "CK-MB, Mb, cTnI, GPBB and H-FABP" used in this example were purchased from abcam company.

[0063] In the present embodiment, the formula of described various solutions is as follows:

[0064] 1.50mM MES buffer (pH5.0) formula (250ml):

[0065] Reagent

source

Final concentration

Dosage per 250ml

MES

(2[N-Morpholino]

ethanesulfonic

acid)

Sigma M-2933

0.05M

2.44g

5MNaOH

Fisher SS256-500

---

5 drops

[0066] 2. PBS formula:

[0067] Reagent

catalog...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a liquid-phase chip for the early diagnosis of myocardial infarction, which mainly comprises coated microspheres, wherein, the coated microspheres comprise the microspheres which are coated by CK-MB capture antibodies, the microspheres which are coated by Mb capture antibodies, the microspheres which are coated by cTnI capture antibodies, the microspheres which are coated by GPBB capture antibodies, the microspheres which are coated by H-FABP capture bodies, detection antibodies which are respectively labeled by biotin, and a streptavidin phycoerythrin. The liquid-phase chip for the early diagnosis of the myocardial infarction which is provided by the invention has the advantages of high detection efficiency, a small number of the needed samples, strong specificity, high sensitivity, etc. At the same time, all the myocardial damage markers can be freely combined, thus the usage is convenient. Moreover, all the reactions are in the liquid-phase environment, which is better for keeping the natural conformation of the protein, and leads the reaction of a probe and an object to be detected to be faster and more complete, thus greatly improving the detection sensitivity and the linear range.

Description

technical field [0001] The invention relates to medical in vitro diagnostic technology, in particular to a liquid phase chip for early diagnosis of acute myocardial infarction and a preparation method thereof. Background technique [0002] Cardiovascular disease has developed into one of the main causes of death in our country, and the highest fatality rate is acute myocardial infarction (AMI). When acute myocardial infarction occurs, the acute infarction of the coronary artery will cause persistent and severe myocardial ischemia, resulting in necrosis of part of the myocardium. It is a very serious type of coronary heart disease, and the current case fatality rate can reach 10% to 15%. Even the survivors in the acute phase, due to myocardial necrosis, impaired blood drainage function, their long-term prognosis is still seriously affected. The incidence, severity and prognosis of the three major complications of acute myocardial infarction (such as malignant arrhythmia, hea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/546G01N33/533
Inventor 许嘉森林一群
Owner SUREXAM BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap