Myocardial infarct early diagnosis liquid phase chip and method for producing the same

Abstract

The invention discloses a liquid-phase chip for the early diagnosis of myocardial infarction, which mainly comprises coated microspheres, wherein, the coated microspheres comprise the microspheres which are coated by CK-MB capture antibodies, the microspheres which are coated by Mb capture antibodies, the microspheres which are coated by cTnI capture antibodies, the microspheres which are coated by GPBB capture antibodies, the microspheres which are coated by H-FABP capture bodies, detection antibodies which are respectively labeled by biotin, and a streptavidin phycoerythrin. The liquid-phase chip for the early diagnosis of the myocardial infarction which is provided by the invention has the advantages of high detection efficiency, a small number of the needed samples, strong specificity, high sensitivity, etc. At the same time, all the myocardial damage markers can be freely combined, thus the usage is convenient. Moreover, all the reactions are in the liquid-phase environment, which is better for keeping the natural conformation of the protein, and leads the reaction of a probe and an object to be detected to be faster and more complete, thus greatly improving the detection sensitivity and the linear range.

Description

technical field [0001] The invention relates to medical in vitro diagnostic technology, in particular to a liquid phase chip for early diagnosis of acute myocardial infarction and a preparation method thereof. Background technique [0002] Cardiovascular disease has developed into one of the main causes of death in our country, and the highest fatality rate is acute myocardial infarction (AMI). When acute myocardial infarction occurs, the acute infarction of the coronary artery will cause persistent and severe myocardial ischemia, resulting in necrosis of part of the myocardium. It is a very serious type of coronary heart disease, and the current case fatality rate can reach 10% to 15%. Even the survivors in the acute phase, due to myocardial necrosis, impaired blood drainage function, their long-term prognosis is still seriously affected. The incidence, severity and prognosis of the three major complications of acute myocardial infarction (such as malignant arrhythmia, hea...

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Problems solved by technology

[0012] The purpose of the present invention is to provide an early diagnosis of myocardial infarction that detects multiple myocardial injury markers synchronously in view of the defects that the sensitivity and specificity of the current acute myocardial infarction detection method are not high and one reaction can only detect one myocardial marker liquid chip

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