Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for measuring protein in sludge according to coomassie brilliant blue method

A Coomassie brilliant blue method and protein technology, applied in the field of protein extraction and determination in sludge, can solve the problems of nitrogen and phosphorus removal, which have not been fundamentally solved, and achieve the effect of easy operation, good parallelism and stable color

Inactive Publication Date: 2014-03-26
BEIJING UNIV OF TECH
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the country has stepped up efforts to control the water environment, and the sewage treatment has been greatly improved. However, the water pollution problems faced have not been fundamentally resolved, the most important of which is the removal of nitrogen and phosphorus.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring protein in sludge according to coomassie brilliant blue method
  • Method for measuring protein in sludge according to coomassie brilliant blue method
  • Method for measuring protein in sludge according to coomassie brilliant blue method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The nitrosated granular sludge and MBR activated sludge on the 10th day, the 20th day and the 40th day were taken as samples, and the protein content was measured.

[0021] Sludge pretreatment: place the sample in a 50ml centrifuge tube; take another centrifuge tube, add tap water to balance the sludge sample, and centrifuge at 8000r / min centrifuge for 15min at room temperature; pour off the supernatant, add 0.05 mol / L Na 3 PO 4 Dilute the sludge to its original volume with a phosphate buffer solution composed of 0.15 mol / L NaCl and a pH value of 7; then shake the sludge to disperse it and ultrasonicate it for 3 minutes.

[0022] Protein extraction: Put the pretreated nitrosation granular sludge and MBR sludge in a water bath at 80°C, heat for 30 minutes, and shake the sludge every 10 minutes; then cool and balance; Centrifuge for 15 minutes, take the supernatant to measure the protein content, and measure the MLSS and MLVSS of the remaining sludge.

[0023] Determin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method for measuring protein in sludge according to the coomassie brilliant blue method belongs to the field of influencing factors in municipal sewage treatment. After being combined with protein, coomassie brilliant blue G-250 becomes cyan, the protein-pigment combined material is highest in light absorption value under a 595nm wavelength condition, and the light absorption value is in direct proportion to protein content, the method can be used for quantitative measurement of protein. The method particularly relates to the following steps: reagent preparation, standard curve measuring, sludge pretreatment, protein extraction and measuring, and the like. The method is a simple and efficient method for measuring the protein content in sewage.

Description

technical field [0001] The invention belongs to the field of urban domestic sewage treatment and regeneration, and in particular relates to a method for extracting and measuring protein in sludge. Background technique [0002] With the acceleration of urbanization and industrialization in our country, water pollution and ecological environment continue to deteriorate, which has seriously affected people's production and life. In recent years, the state has intensified its efforts to control the water environment, and sewage treatment has been greatly improved. However, the water pollution problems it faces have not been fundamentally resolved, the most important of which is the removal of nitrogen and phosphorus. [0003] The denitrification and phosphorus removal of sewage mainly adopts activated sludge method and biofilm method, and the parameters to control the effect of sewage treatment mainly include T, pH, DO, SRT, etc., and with the deepening of research, researchers ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 李冬张玉龙范丹张肖静梁瑜海何永平张翠丹吴青门绚杨胤苏庆岭周元正曾辉平张杰
Owner BEIJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com