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Foam separation method for beta-glucanase in fermentation liquor

A glucanase and foam separation technology, applied in the directions of microorganism-based methods, glycosylases, biochemical equipment and methods, etc., can solve the problems of difficult separation, product inhibition, low utilization rate of nutrients, etc. High resolution, reduced effect of denaturation, reduced effect of contamination

Pending Publication Date: 2022-01-25
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of product inhibition, difficult separation and low utilization rate of nutrients in the production process of β-glucanase existing in the prior art, to provide the foaming property of β-glucanase itself, and to explore from Foam separation and harvesting technology of β-glucanase in fermentation broth, and a foam separation method of β-glucanase in fermentation broth with optimized process conditions

Method used

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  • Foam separation method for beta-glucanase in fermentation liquor
  • Foam separation method for beta-glucanase in fermentation liquor
  • Foam separation method for beta-glucanase in fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This example is to change the aeration rate in the fermentation broth, fix other experimental factors, compare the recovery rate and enrichment ratio of β-glucanase in the shake flask culture solution under different aeration rates, and determine the best separation and harvest of shake flask β - Aeration rate of dextranase.

[0056] Five groups of foam separation experiments were designed, the gradient of ventilation rate was 200mL / min, 200mL / min, 400mL / min, 600mL / min, 800mL / min, 1000mL / min were selected respectively, other conditions were determined as follows: liquid volume 100mL, initial enzyme The concentration is 100 μg / mL, the surfactant concentration is 0.4 mg / mL, the pH value of the solution is 6, and the collection time is 10 min. The result is as figure 2 shown.

[0057] According to the experimental results, the enrichment ratio E of β-glucanase decreases with the increase of the ventilation rate, and the enrichment ratio E has a maximum value of 1.69 whe...

Embodiment 2

[0059] This example is to change the liquid volume in the foam separation system, fix other experimental factors, compare the recovery rate and enrichment ratio of β-glucanase in the shake flask culture solution under different liquid volumes, and determine the best separation and recovery Filling volume of shake flask β-glucanase.

[0060] Five groups of foam separation experiments were designed, and the filling volumes were 50mL, 150mL, 200mL, 250mL, and 300mL, and the other conditions were determined as follows: the ventilation rate was 400mL / min, the initial enzyme concentration was 100μg / mL, and the surfactant concentration was 0.4mg / mL , the pH of the solution was 6, and the collection time was 10 minutes. The result is as image 3 shown.

[0061] From the experimental results, the enzyme activity recovery rate R of β-glucanase increases with the increase of the filling volume, and has a maximum value of 35.76% when the filling volume is 300mL. The enrichment ratio E ...

Embodiment 3

[0063] This example is to change the initial enzyme concentration in the foam separation system, fix other experimental factors, compare the recovery rate and enrichment ratio of β-glucanase in the shake flask culture solution under different initial enzyme concentrations, and determine the best separation and recovery Initial enzyme concentration of shake flask β-glucanase.

[0064] Five groups of foam separation experiments were designed, the initial enzyme concentration gradient was 25 μg / mL, 50 μg / mL, 75 μg / mL, 100 μg / mL, 125 μg / mL, 150 μg / mL were selected respectively, other conditions were determined as follows: ventilation rate 400 mL / min, The filling volume is 100mL, the surfactant concentration is 0.4mg / mL, the pH value of the solution is 6, and the collection time is 10min. The result is as Figure 4 shown.

[0065] From the results, the enzyme activity recovery R of β-glucanase showed a trend of first increasing and then decreasing with the increase of the initial...

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Abstract

The invention discloses a foam separation method for beta-glucanase in fermentation liquor and belongs to the technical field of foam separation. The method comprises the steps: carrying out fermentation in a fermentation tank to produce beta-glucanase, preparing beta-glucanase solutions with different concentrations from beta-glucanase fermentation liquor, and measuring the foaming capacity and foam stability of the beta-glucanase solutions; checking the air tightness of a foam separation device, and introducing the fermentation liquor into an air floatation pipe of the foam separation device; and opening a gas cylinder main valve and a pressure reducing valve, adjusting a rotor flow meter, adjusting a separation gas speed to a required value, introducing air into the fermentation liquor, enabling the gas to reach the bottom of the floatation pipe, uniformly distributing the gas on a gas distributor, conveying the gas upwards, driving a solution solute and bubbles in a separation foam generating pipe to be discharged from the top end of the air floatation pipe, collecting the solution solute and the bubbles into a foam collecting beaker, measuring a concentration and enzyme activity of beta-glucanase in foam liquid, and calculating the recovery rate and enzyme activity enrichment ratio of foam separation. The method has the advantages of low cost, mild conditions and simplicity and convenience in operation, and the problems of product inhibition, difficulty in separation, low nutrient utilization rate and the like are solved.

Description

technical field [0001] The invention belongs to the technical field of foam separation, and in particular relates to a foam separation method for beta-glucanase in a fermented liquid using SDS as a foaming agent. Background technique [0002] β-glucanase can catalyze the decomposition of β-glucan and exists as a structural element in the cell walls of yeast, fungi and cereals. β-glucanase can be divided into four categories, among which β-1,3-1,4-glucanase (Yang S Q, Yan Q J, Jiang Z Q, et al. Biochemical characterization of a novel thermostablebeta-1,3-1,4-glucanase (Lichenase) from Paecilomyces thermophila[J].Journal ofAgricultural and Food Chemistry, 2008,56(13):5345-5351), which has strict substrate specificity, Therefore, it is widely used in food, detergent, animal feed and other industries. The common extraction methods of β-glucanase are: plant extraction (Sun Yuying, Wang Ruiming. Research progress of β-glucanase [J]. Journal of Shandong Commercial Vocational and ...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12R1/84
CPCC12N9/2448C12Y302/01073
Inventor 邵文尧秦宇航林滢陈国玺
Owner XIAMEN UNIV
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