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Determination method for soluble protein content in tobacco or product thereof

A technology of protein content and determination method, which is applied in the field of determination of soluble protein content in tobacco or its products, can solve the problems of poor result accuracy and troublesome pre-treatment, and achieve the effects of high accuracy, simple operation and convenient recovery

Inactive Publication Date: 2014-05-28
CHINA TOBACCO ANHUI RECONSTITUTED TOBACCO SCI & TECH CO LTD
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Problems solved by technology

This method is calculated based on empirical values, the accuracy of the result is slightly worse, and the pre-processing is more troublesome

Method used

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  • Determination method for soluble protein content in tobacco or product thereof
  • Determination method for soluble protein content in tobacco or product thereof
  • Determination method for soluble protein content in tobacco or product thereof

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Embodiment Construction

[0026] The invention will be further described below through specific embodiments. The specific embodiments described here are only for explaining the invention, and are not intended to limit the protection scope of the present invention.

[0027] A method for determining the content of soluble protein in tobacco or its products, comprising the following specific steps:

[0028] (1) Weigh 0.25-1.0 g of tobacco sample or tobacco product sample, add 25-100 mL of distilled water to dissolve, then ultrasonically shake for 20-45 min, and centrifuge at 3000-10000 rpm for 5-20 min, and the supernatant The solution is filtered into the sample cup through a medium-speed quantitative ash-free filter paper to obtain the sample solution to be tested;

[0029] (2) Weigh 1.0 g of bovine serum albumin and dissolve it in distilled water, dilute to 100 mL, and filter through medium-speed quantitative ashless filter paper to obtain 10 mg / mL bovine serum albumin stock solution, and then dilute ...

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Abstract

The invention discloses a determination method for soluble protein content in tobacco or a product thereof. The determination method comprises the following steps: weighing a tobacco sample or a sample of its product, dissolving the sample with water and carrying out ultrasonic concussion and centrifugation so as to obtain a sample solution to be detected; dissolving bovine serum albumin in water to prepare a bovine serum albumin stock solution and diluting the bovine serum albumin stock solution to obtain bovine serum albumin standard solutions with different concentrations; dissolving Coomassie brilliant blue G-250 in 90% ethanol, adding phosphoric acid with mass concentration of 850 g / L, allowing a constant volume to be obtained through addition of water and then adding Brij-35 to prepare a Coomassie brilliant blue G-250 standard solution; and combining the bovine serum albumin standard solutions respectively with the Coomassie brilliant blue G-250 standard solution and the sample solution to be detected, sequentially introducing samples of all the reagents by running the program of a continuous flow analyzer and detecting soluble protein content in the sample to be detected under the condition of wave length of 595 nm. The method can directly detect soluble protein content without artificial comparison with a standard curve and has the advantages of simple operation, a high degree of automation, convenient sample recovery and high accuracy and repeatability.

Description

technical field [0001] The invention relates to a detection technology of tobacco, in particular to a method for measuring the content of soluble protein in tobacco or its products. technical background [0002] Among the many components of tobacco leaves, the protein content is the most abundant. For example, the protein content in Burley tobacco is as high as 20.48%. The protein in tobacco leaves is divided into soluble protein and insoluble protein. About half of the soluble proteins are chloroplast proteins (Fraction I protein, FI protein), and the other half are complexes of other soluble proteins (Fraction II protein, FII protein). The research results show that the content of soluble protein in plant leaves is the highest in tobacco leaves, and the content of various essential amino acids in FI protein is not only higher than the content standards of essential amino acids in protein products formulated by the World Food and Agriculture Organization (FAO), among which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 苏瑶赵春雷陶杰高军丁嘉珅章存勇
Owner CHINA TOBACCO ANHUI RECONSTITUTED TOBACCO SCI & TECH CO LTD
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