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Process for screening of microorganism flocculator producing bacteria

A technology of a microbial flocculant and a screening method, which is applied in the field of screening of bacteria producing microbial flocculants, can solve the problems of uncertain screening targets, low screening efficiency and large workload, and achieves high screening efficiency, small workload and short cycle. Effect

Inactive Publication Date: 2005-11-09
PENGZE MICROORGANISM SCI & TECH KUNMING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of screening microbial flocculant-producing bacteria is mainly to obtain pure cultures through plate separation, and then culture each pure culture in shake flasks to determine whether the fermentation broth has flocculation activity to determine flocculant-producing bacteria. There is a screening Uncertain target, heavy workload, long cycle, low screening efficiency and other disadvantages

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Screening of flocculating active bacteria

[0015] Take 1000ml bacterial culture medium (g / L: sucrose 10, K 2 HPO 4 1.5, FeCl 3 0.002, CaCO 3 1.5, yeast extract 0.5, agar 20, water 1000ml, pH7.0~7.5) In a container, add 10mg Coomassie Brilliant Blue and Congo Red respectively, shake well, sterilize at 121℃ for 20min., make a flat plate, and coat differently. The diluted soil suspension is cultured at 25-30°C until a single colony is formed on the plate. Pick the red colonies on the blue background and insert 50ml liquid medium (g / L: sucrose 10, K 2 HPO 4 1.5, FeCl 3 0.002, CaCO 3 1.5. Yeast extract 0.5, pH 7.0-7.5), cultured at 25-30°C for 4 days and then used kaolin suspension to measure the flocculating activity of the fermentation broth. As a result, 99.6% of the strains had flocculating activity.

Embodiment 2

[0016] Example 2: Screening of flocculating active actinomycetes

[0017] Take 1000ml actinomycete culture medium (g / L: soluble starch 20, KNO 3 1, NaCl 0.5, K 2 HPO 4 0.5, MgSO 4 0.5, FeSO 4 0.01, agar 20, 1000ml of water, pH 7.2-7.4) in a container, add 10mg of Coomassie Brilliant Blue and Congo Red respectively, shake well, sterilize at 121°C for 30min., make a flat plate, and coat soil with different dilutions The suspension was cultured at 25°C until a single colony was formed on the plate. Pick the red colonies on the blue background and insert 50ml liquid medium (g / L: soluble starch 20, KNO 3 1, NaCl 0.5, K 2 HPO 4 0.5, MgSO 4 0.5, FeSO 4 0.01, 1000 ml of water, pH 7.2-7.4), cultured at 25°C for 10 days and then used kaolin suspension to measure the flocculating activity of the fermentation broth. As a result, 87.9% of the strains had flocculating activity.

Embodiment 3

[0018] Example 3: Screening of flocculating active yeast

[0019] Take 1000ml yeast culture medium [g / L: yeast extract 3, malt extract 3, peptone 5, glucose 20, agar 20, pH 5.3 (after autoclaving, adjust with hydrochloric acid or phosphoric acid, add broad-spectrum antibiotics to inhibit bacteria) ]In a container, add 10mg of Coomassie Brilliant Blue and Congo Red respectively, shake well, sterilize at 110°C for 30min., make a flat plate, spread soil suspension of different dilutions, culture at 25°C until a single colony is formed on the plate . Pick the red colonies on the blue background and insert 50ml liquid medium [g / L: yeast extract 3, malt extract 3, peptone 5, glucose 20, agar 20, pH 5.3 (after autoclaving with hydrochloric acid or phosphoric acid) Adjustment)], the kaolin suspension was used to measure the flocculating activity of the fermentation broth after culturing at 25°C for 4 days, and it was found that 90.5% of the strains had flocculating activity.

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Abstract

A method for screening the microbial flocculant generating bacteria features that the Congo red (C32H22N6Na2O6S2) and the Coomassie brilliant blue are simultaneously added to the culture medium for selectively screening the flocculant generating bacteria from different specimens.

Description

Technical field [0001] The invention relates to a method for screening microbial flocculant producing bacteria. Background technique [0002] With the development of the global economy and the progress of industrialized production, the living standards of mankind have been greatly improved, but the environment on which mankind depends for survival has been increasingly destroyed and the laws of the environment have become disordered. The severe situation of water scarcity and increased pollution has promoted the research and application of water treatment agents. In the history of the development of water treatment agent flocculation technology, inorganic salt flocculants (such as aluminum sulfate, aluminum chloride, ferric chloride, etc.) were developed in the 1960s, and their treatment effects were not ideal; and then the polymer polymerized inorganic salt type flocculant was developed Agents (such as polyaluminum chloride, polyferric sulfate, etc.) have received good results i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 李文鹏朱光勇潘基敏王建明
Owner PENGZE MICROORGANISM SCI & TECH KUNMING
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