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Detection method of lactobacillus micro integral membrane protein active fragments

A technology of integral membrane protein and detection method, which is applied in the field of detection of active fragments of integral membrane protein of Lactobacillus, and can solve the problem of small molecules

Active Publication Date: 2012-03-14
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are few studies on the active fragment of the integral membrane protein of Lactobacillus, and the active fragment is a small fragment (Micro Integral Membrane Protein, MIMP) with adhesion activity, which is small in size, has high potency and specificity, and has application value. Larger; in addition, because of its unique hydrophilic and hydrophobic structure and its biological effects, it can make up for the defects of Lactobacillus translocation and antibiotic resistance, and has important clinical application value

Method used

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  • Detection method of lactobacillus micro integral membrane protein active fragments
  • Detection method of lactobacillus micro integral membrane protein active fragments
  • Detection method of lactobacillus micro integral membrane protein active fragments

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Embodiment Construction

[0027] The inventors have found through long-term research that the adhesion structure region of lactobacillus is located in the integral membrane protein on the surface of the bacterium, which is a small fragment with adhesion.

[0028] The raw material is Lactobacillus plantarum (LP for short, CGMCC No.1258), and the detection method of the active fragment MIMP of Lactobacillus plantarum in the present invention is specifically introduced as follows:

[0029] Lactobacillus (CGMCC No.1258) Surface Integral Membrane Protein Sequence (SEQ ID No: 1):

[0030] 1 mktvrkiplv vwytamfvvi atitysglfl agrtliwevd giaqhfpill efqrilqhhp

[0031] 61 qqlfswswnl glgadqlttf syyvvgdpfn ylvafvsrah lewayqalil lrlyfvglaf

[0032]121 lgfsrqfkfk rvsqligalt ytftaytfyv gmhhpffllp miwfpllcwa iervlrgrhw

[0033] 181 lplslitavv ilsnfyfayl lalgglvyal vrfwsrrrdh ltmrsfgqlf wrllvavglg

[0034] 241 vtmagillvp tllamltatr asfnfanglt sypinyyvnl pnrlltnggs vqywvtlgls

[0035] 301 sisfiaiiiyt lrhfrrywvl nwvlv...

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Abstract

The invention relates to a detection method of lactobacillus micro integral membrane protein active fragments. According to the invention, first, 6 N-terminal deleted or C-terminal deleted mutant proteins are established; mucin marked by HRP is adopted as a marked first antibody, and western bolt (WB) and Coomassie brilliant blue staining detections are carried out; WB results and Coomassie brilliant blue staining results are compared, such that the positions of the active fragments in the lactobacillus micro integral membrane protein sequence are obtained.

Description

technical field [0001] The invention relates to a detection method for an active fragment of an integral membrane protein, in particular to a detection method for an active fragment of a lactobacillus integral membrane protein. Background technique [0002] Probiotics refer to live bacterial preparations and their metabolites that improve the host's micro-ecological balance and play a beneficial role in improving the host's health level and health status. They are widely found in every corner of the earth and are beneficial to humans and animals. Bacteria, they can be taken directly as food additives to maintain the balance of intestinal flora, and the adhesion and colonization in the intestinal tract can inhibit the damage of pathogenic bacteria, which is of great significance for protecting the integrity of the intestinal mucosa; since the 1990s Since the beginning, all kinds of "probiotics" health care products have become popular all over the world. At the same time, the...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569G01N27/447C12N15/31C12N15/63C07K14/195
Inventor 秦环龙刘志华
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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