Detection method of lactobacillus micro integral membrane protein active fragments

A technology of integral membrane protein and detection method, which is applied in the field of detection of active fragments of integral membrane protein of Lactobacillus, and can solve the problem of small molecules

Active Publication Date: 2012-03-14
SHANGHAI SIXTH PEOPLES HOSPITAL
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are few studies on the active fragment of the integral membrane protein of Lactobacillus, and the active fragment is a small fragment (Micro Integral Membrane Protein, MIMP) with adhesion activity, which is small in size, has high potency and specifici

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of lactobacillus micro integral membrane protein active fragments
  • Detection method of lactobacillus micro integral membrane protein active fragments
  • Detection method of lactobacillus micro integral membrane protein active fragments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The inventors have found through long-term research that the adhesion structure region of lactobacillus is located in the integral membrane protein on the surface of the bacterium, which is a small fragment with adhesion.

[0028] The raw material is Lactobacillus plantarum (LP for short, CGMCC No.1258), and the detection method of the active fragment MIMP of Lactobacillus plantarum in the present invention is specifically introduced as follows:

[0029] Lactobacillus (CGMCC No.1258) Surface Integral Membrane Protein Sequence (SEQ ID No: 1):

[0030] 1 mktvrkiplv vwytamfvvi atitysglfl agrtliwevd giaqhfpill efqrilqhhp

[0031] 61 qqlfswswnl glgadqlttf syyvvgdpfn ylvafvsrah lewayqalil lrlyfvglaf

[0032]121 lgfsrqfkfk rvsqligalt ytftaytfyv gmhhpffllp miwfpllcwa iervlrgrhw

[0033] 181 lplslitavv ilsnfyfayl lalgglvyal vrfwsrrrdh ltmrsfgqlf wrllvavglg

[0034] 241 vtmagillvp tllamltatr asfnfanglt sypinyyvnl pnrlltnggs vqywvtlgls

[0035] 301 sisfiaiiiyt lrhfrrywvl nwvlv...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention relates to a detection method of lactobacillus micro integral membrane protein active fragments. According to the invention, first, 6 N-terminal deleted or C-terminal deleted mutant proteins are established; mucin marked by HRP is adopted as a marked first antibody, and western bolt (WB) and Coomassie brilliant blue staining detections are carried out; WB results and Coomassie brilliant blue staining results are compared, such that the positions of the active fragments in the lactobacillus micro integral membrane protein sequence are obtained.

Description

technical field [0001] The invention relates to a detection method for an active fragment of an integral membrane protein, in particular to a detection method for an active fragment of a lactobacillus integral membrane protein. Background technique [0002] Probiotics refer to live bacterial preparations and their metabolites that improve the host's micro-ecological balance and play a beneficial role in improving the host's health level and health status. They are widely found in every corner of the earth and are beneficial to humans and animals. Bacteria, they can be taken directly as food additives to maintain the balance of intestinal flora, and the adhesion and colonization in the intestinal tract can inhibit the damage of pathogenic bacteria, which is of great significance for protecting the integrity of the intestinal mucosa; since the 1990s Since the beginning, all kinds of "probiotics" health care products have become popular all over the world. At the same time, the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/569G01N27/447C12N15/31C12N15/63C07K14/195
Inventor 秦环龙刘志华
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap