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Kit for pathologic diagnosis of tumors and method for staining tissue sections

A technology for pathological diagnosis and tissue sectioning, applied in the field of molecular biology, can solve the problems of high price, inactivation and high cost, and achieve the effects of less workload, rapid detection and easy operation.

Active Publication Date: 2013-08-14
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since these methods use natural peroxidase and organic fluorescent dyes, natural peroxidase is easily denatured and inactivated, and it is difficult to purify, expensive, inconvenient to store and use, and high in cost.
The fluorescence of organic fluorescent dyes is easy to be extracted, resulting in inaccurate diagnostic results such as false negatives in the test results

Method used

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  • Kit for pathologic diagnosis of tumors and method for staining tissue sections
  • Kit for pathologic diagnosis of tumors and method for staining tissue sections
  • Kit for pathologic diagnosis of tumors and method for staining tissue sections

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preparation example Construction

[0041] This embodiment also provides a method for preparing a probe for tumor pathological diagnosis, including the following steps:

[0042] Step S110, mixing the chloroauric acid solution with a concentration of 1-100 mmol / L and the bovine serum albumin or human serum albumin solution with a concentration of 1-500 mg / mL evenly, and then adding a NaOH solution with a concentration of 0.1-10 mol / L , and incubated in a shaker at 0-100° C. for 3-100 hours to obtain gold nanoclusters, wherein the molar ratio of NaOH to chloroauric acid is 1:1-1:100.

[0043] In this embodiment, bovine serum albumin is preferred to prepare gold nanoclusters, because bovine serum albumin has the advantages of non-toxicity, good biocompatibility, and relatively cheap price.

[0044] Step S120 , mixing the gold nanoclusters with the azide reagent pretreated by NHS at a molar ratio of 1:1 to 1:100 to obtain gold nanoclusters with azide groups modified on the surface.

[0045] In this embodiment, the ...

Embodiment 1

[0065] The antibody is an anti-HER2 protein antibody, and the paraffin tumor tissue section is human breast cancer cell MCF7

[0066] 1. Preparation of probes for pathological diagnosis of tumors

[0067] 1. Preparation of gold nanoclusters: at 37°C, add 0.5mL of chloroauric acid solution with a concentration of 50mmol / L to 0.5mL of BSA solution with a concentration of 200mg / mL and mix well; then add 0.2mL with a concentration of 1mol / L of NaOH solution to obtain a mixed solution; the mixed solution was placed in a shaker and incubated at 37°C for 10 hours to obtain gold nanoclusters.

[0068] 2. Azido group modification of gold nanoclusters: mix the above gold nanoclusters with the azide reagent at a molar ratio of 1:1 to obtain gold nanoclusters with azide groups on the surface, wherein the azide reagent Pre-treated by the NHS.

[0069] 3. Alkynyl modification of anti-HER2 protein antibody: Add DBCO-sulfo-NHS Ester alkynylation reagent to anti-HER2 protein antibody and re...

Embodiment 2

[0094] The antibody is an anti-HER2 protein antibody, and the paraffin tumor tissue section is human liver cancer cell HepG2.

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Abstract

The invention relates to a kit for the pathologic diagnosis of tumors. The kit comprises a probe for the pathologic diagnosis of the tumors, wherein the probe comprises a gold nanoparticle cluster, an antibody of a tumor specific marker and CBB (Coomassie brilliant blue) in the molar ratio of (1-10):(1-100):(1-100); the gold nanoparticle cluster and the antibody of the tumor specific marker are connected in the manner of click chemistry; and the CBB and the gold nanoparticle cluster connected with the antibody of the tumor specific marker are combined in a static manner. The kit has high accuracy on the pathologic diagnosis of the tumors. In addition, the invention also provides a method for staining tissue sections.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a kit for pathological diagnosis of tumors and a method for staining tissue sections using the kit for pathological diagnosis of tumors. Background technique [0002] In the past two decades, the mortality rate of cancer has risen sharply. Among middle-aged and middle-aged people aged 35 to 59, tumors have ranked first among various causes of death. Statistics show that the incidence of cancer in my country is about 2 million per 100,000 people, with more than 2.2 million new cases each year and more than 6 million patients being treated. Tumor has become a major cause of the loss of the best labor force and the rise of medical expenses in our country. Although some chemical drugs to prevent tumors have been developed, there is still no primary preventive measure to effectively control tumors. Therefore, early diagnosis is still a basic strategy for tumor prevention ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N1/30G01N21/64
Inventor 蔡林涛胡德红盛宗海方胜涛高笃阳王亚楠龚萍张鹏飞
Owner SHENZHEN INST OF ADVANCED TECH
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