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Application of epalrestat in preparation of pancreatic cancer drugs and method for verifying inhibiting effect on exosome secretion of pancreatic cancer cells

A technology based on pancreatic cancer cells and epalrestat, which can be used in drug combinations, pharmaceutical formulations, biomaterial analysis, etc. It can solve problems such as high investment risks and no clinical trials, and achieve great application potential and inhibit exosomes. secretory effect

Inactive Publication Date: 2019-07-26
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Abstract
  • Description
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Problems solved by technology

Although these drugs can inhibit the production and release of exosomes, clinical trials have not yet been carried out, and the development of a new drug requires high investment and unknown risks

Method used

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  • Application of epalrestat in preparation of pancreatic cancer drugs and method for verifying inhibiting effect on exosome secretion of pancreatic cancer cells
  • Application of epalrestat in preparation of pancreatic cancer drugs and method for verifying inhibiting effect on exosome secretion of pancreatic cancer cells
  • Application of epalrestat in preparation of pancreatic cancer drugs and method for verifying inhibiting effect on exosome secretion of pancreatic cancer cells

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Embodiment Construction

[0023] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail with reference to the drawings and specific embodiments.

[0024] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", " The orientation or positional relationship indicated by "front", "rear", etc. is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, are constructed and operate in a particular orientation and therefore are not to be construed as limiting the invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and shou...

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Abstract

The invention provides an application of epalrestat in preparation of pancreatic cancer drugs. The pancreatic cancer drugs are used for inhibiting exosome secretion of pancreatic cancer cells. A pharmaceutical composition for treating pancreatic cancer contians epalrestat. The pharmaceutical composition is used for inhibiting exosome secretion of pancreatic cancer cells. The embodiment of the invention also provides a method for verifying the inhibition effect of epalrestat on exosome secretion by pancreatic cancer cells. The method comprises the following steps: extracting cell supernatant exosomes by using a low-temperature ultracentrifugation method; lysing the collected exosomes, using a BCA kit to quantify protein, and using the measured amount of the protein to reflect the amount ofthe exosomes; using a transmission electron microscope to verify a double-layer lipid membrane wrapped cup-shaped structure of the exosomes; detecting exosome protein concentration by protein polyacrylamide gel electrophoresis with Coomassie brilliant blue. The invention provides a novel application of epalrestat, namely the inhibition of exosome secretion. The epalrestat has great application potential in clinical tumor treatment.

Description

technical field [0001] The invention belongs to the application technical field of epalrestat, and specifically relates to an application of epalrestat in the preparation of pancreatic cancer drugs and a method for verifying its inhibitory effect on secretion of exosomes by pancreatic cancer cells. Background technique [0002] The special tumor microenvironment plays a key role in the occurrence, development, metastasis and drug resistance of pancreatic cancer. Pancreatic cancer aids its progression by secreting exosomes to shape the microenvironment of itself and other organs. Compared with normal cells and other tumors, pancreatic cancer cells secrete more exosomes, and highly malignant pancreatic cancer cells can transfer their cancerous characteristics to low-grade cancer cells through exosomes, promoting their proliferation, Migration and invasion to accelerate disease progression. Exosomes derived from pancreatic cancer can also be taken up by Kupffer cells in the l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/426A61P35/00G01N33/50G01N33/535G01N33/569G01N33/574
CPCA61K31/426A61P35/00G01N33/5011G01N33/507G01N33/535G01N33/56966G01N33/57438G01N33/5076G01N33/6839G01N2800/52G01N23/2251G01N1/30
Inventor 肖明兵焦钰洁季洁范义辉邰伯军谭忠华倪温慨陈晓君纪易斐
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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