Extraction method and application of peanut leaf soluble protein
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An extraction method, peanut leaf technology, which is applied in the field of extraction of soluble protein, can solve the problems of waste of resources, etc., and achieve the effects of improving the extraction rate, being easy to implement, and simplifying the extraction process
Inactive Publication Date: 2018-11-27
LIAONING UNIVERSITY
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Embodiment 1
[0031] (1) A kind of extraction method of peanut leaf soluble protein
[0032] 1) Use peanut leaves as raw materials, dry them in the air, grind the washed and dried peanut leaves with a powder machine, and sieve to obtain peanut leaf powder.
[0033] 2) Take 40 g of the sieved peanut leaf powder, add 800 mL of distilled water, stir well to mix the leaf powder evenly, then adjust the pH to 9.0, stir and extract for 40 minutes to obtain a peanut leaf powder extraction solution.
[0034] 3) Centrifuge the extraction solution of peanut leaf powder for 20 min under the condition of 5000 r / min, separate the supernatant, and discard the precipitate.
[0035] 4) Adjust the pH of the obtained supernatant to 2.0.
[0036] 5) Centrifuge the pH-adjusted solution for 30 minutes at a speed of 5000 r / min to obtain a precipitate, which is peanut leaf soluble protein.
[0037] 6) Put the precipitate obtained by centrifugation into a beaker, add about 100 mL of distilled water, adjust the pH...
Embodiment 2
[0065] Example 2 Determination of Antioxidant Properties of Peanut Leaf Soluble Protein
[0066] (1) Preparation of Peanut Leaf Soluble Protein
[0067] 1) Use peanut leaves as raw materials, dry them in the air, grind the washed and dried peanut leaves with a powder machine, and sieve to obtain peanut leaf powder.
[0068] 2) Take 40 g of the sieved peanut leaf powder, add 800 mL of distilled water, stir well to mix the leaf powder evenly, then adjust the pH to 9.0, stir and extract for 40 minutes to obtain a peanut leaf powder extraction solution.
[0069] 3) Centrifuge the extraction solution of peanut leaf powder for 20 min under the condition of 5000 r / min, separate the supernatant, and discard the precipitate.
[0070] 4) Adjust the pH of the resulting supernatant to 2.0.
[0071] 5) Centrifuge the pH-adjusted solution for 30 minutes at a speed of 5000 r / min to obtain a precipitate, which is peanut leaf soluble protein.
[0072] 6) Put the precipitate obtained by cent...
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Abstract
The invention discloses an extraction method and application of a peanut leaf soluble protein. The extraction method comprises the following steps: (1) carrying out sun-drying and pulverization on fresh peanut leaves as a raw material; (2) adding distilled water, adjusting the pH value, and carrying out extraction; (3) centrifuging extracted solution, separating to obtain supernate, and removing precipitates; (4) adjusting the pH value of the obtained supernate; (5) centrifuging the solution after the pH value is adjusted, and precipitating, so as to separate the peanut leaf soluble protein; (6) carrying out redissolution on the obtained precipitates with distilled water, and adjusting the pH value; and (7) after the redissolution is finished, carrying out vacuum freeze drying on the protein solution, so as to obtain peanut leaf soluble protein. According to the extraction method, the peanut leaf soluble protein is extracted by virtue of an alkali solution and acid precipitation method, the extraction rate of the peanut leaf protein is high and is 44.59%, and the extraction method is easy in operation and implementation. A Coomassie brilliant blue analysis and antioxidant activitydetermination result presents that the purity of the peanut leaf soluble protein is 63.83%, and the peanut leaf soluble protein has good antioxidant activity.
Description
technical field [0001] The invention belongs to the field of extracting functional biomacromolecular substances from natural raw materials, and in particular relates to a method for extracting soluble protein from peanut leaves. Background technique [0002] Plant leaf protein is a green protein concentrate extracted from fresh plant stems and leaves after pressing, which is rich in essential amino acids and non-essential amino acids required by the human body, and has rich nutritional value. Plant stem and leaf protein is one of the most renewable protein resources in the world. Its extracts and processed products will be widely used in food, feed, medicine, pesticides, cosmetics and plant growth regulators. Leaf protein can also be further separated It can produce many functional active substances, such as chlorophyll, carotene, pure protein and vitamin E. The research on plant stem and leaf protein products in my country has just started, the industrialized production ha...
Claims
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