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Method for detecting content of protein in fiber

A protein content, fiber technique used in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, analysis by chemically reacting materials, etc.

Inactive Publication Date: 2014-12-03
YANCHENG INST OF IND TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports on the application of Coomassie Brilliant Blue method in textile fiber inspection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the mensuration of protein content in cotton fiber.

[0029] Cotton is a natural cellulose fiber, its main component is cellulose, but the fiber contains a small amount of protein and other companions. The protein content is very low and is affected by many factors, such as: variety, origin, season, maturity, etc. Investigating the content of accompanying substances and impurities in fibers is also an important content of fiber analysis.

[0030] Prepare protein standard solution and Coomassie Brilliant Blue G-250 solution according to steps 1, 2, and 3 in the technical plan, test with a spectrophotometer and draw a standard curve to obtain a linear regression equation Y=0.00645X+0.15730, and the linear correlation coefficient r = 0.9992. Accurately weigh 0.6g of the fiber sample to be tested in a test tube, and dissolve the sample with 1.0mol / L sodium hypochlorite 10mL at 20°C for 30min, so that the protein substances in the fiber are fully dissolved in...

Embodiment 2

[0031] Embodiment 2: the mensuration of protein content in hemp fiber.

[0032] The main component of hemp fiber is cellulose, and the protein content is very low, existing in the form of impurities. Using the test process in Example 1, the protein content in the hemp fiber was tested, and the average protein content was calculated to be 0.055%.

Embodiment 3-6

[0033] Examples 3-6: Determination of protein content in regenerated cellulose.

[0034] The protein content of regenerated cellulose fibers or fabrics should be very low or zero, and the test method is similar to that of cotton and linen fibers. Using the testing procedure in Example 1, several representative regenerated cellulose fibers were tested for protein content.

[0035] Table 2 Protein content in several regenerated cellulose fibers

[0036] Example

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PUM

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Abstract

The invention relates to a method for detecting content of protein in a fiber. A Coomassie brilliant blue approach is applied to quantitative detection on the content of protein in the fiber. Coomassie brilliant blue G-250 is red under a free state; after the G-250 is combined with an alkaline amino acid and an aromatic amino acid residue of the protein in a dilute acid solution, the color becomes blue, the maximum absorption wavelength becomes 595nm from 465nm, the protein ranges from 1 microgram to 1000 micrograms, the absorbance of a protein-pigment conjugate under the wavelength of 595nm is in direct proportion to the content of protein, and the relationship conforms to the Lambert-Beer law, so that the Coomassie brilliant blue can be used for quantitatively determining the content of protein in the fiber. The detection process comprises the following steps: preparing a protein standard solution, preparing a Coomassie brilliant blue solution, drawing a standard curve, obtaining the equation of linear regression and the linear correlation coefficient r, preparing a test product solution, determining the content of protein in a sample, and calculating and analyzing the result.

Description

technical field [0001] The invention relates to a method for detecting protein content in fibers, belonging to the technical field of textile material inspection. Background technique [0002] At present, the components of textile fabrics are becoming more and more complex, and have developed from single and double components to multi-components. The composition of natural fibers in fiber materials is more complicated. Taking cotton as an example, its main component is cellulose, and it also contains a small amount of accompanying organisms such as pentosan, wax, protein, fat, water-soluble substances and ash. How to identify fiber types and the content of each component is also an important content of fiber inspection. Fiber identification includes identification of morphological characteristics and identification of physical and chemical properties. Microscopic observation is commonly used to identify morphological features. There are many methods for identification of p...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78
Inventor 吴焕岭顾东雅
Owner YANCHENG INST OF IND TECH
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