Marine pollutant micro-plastic dyeing method

A dyeing method and marine pollution technology, applied in the field of marine pollutant research, can solve the problems of expensive and extensive research on Nile red

Inactive Publication Date: 2018-10-09
HAINAN TROPICAL OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only reports in the literature that Nile Red can stain microplastics, but Nile Red is expensive and not suitable for extensive research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The marine pollution microplastic dyeing method of embodiment 1, comprises the steps:

[0022] Stain solution preparation: mix Coomassie brilliant blue and methylene blue in ethanol solution at 1:1 equal concentration to form a stain solution, in which the concentration of Coomassie brilliant blue is 0.1g / L, and the concentration of methylene blue is 0.1g / L. in ethanol solution;

[0023] Marine pollutant treatment: pre-select a certain amount of marine pollutants, and then select a mixture containing microplastics from marine pollutants, such as sea sand. Specifically, first collect the sea sand, soak the sample sand with saturated sodium iodide saturated solution, inflate it for more than half an hour, then stop the aeration, let it stand for more than 24 hours, then pour out the supernatant, and filter it with a 0.45 micron organic filter membrane.

[0024] Dyeing treatment: Add dye and water to the filter membrane formed in the previous step to dye the entire filter...

Embodiment 2

[0027] An embodiment of the present invention provides a method for dyeing marine polluted microplastics, which includes the following steps:

[0028] Stain solution preparation: Coomassie brilliant blue and methylene blue were mixed in ethanol solution at a concentration ratio of 1.5:1 to form a stain solution, wherein the concentration of Coomassie brilliant blue was 0.15g / L, and the concentration of methylene blue was 0.1g / L. are in ethanol solution;

[0029] Marine pollutant treatment: pre-select a certain amount of marine pollutants, and then select a mixture containing microplastics from marine pollutants, such as sea sand. Specifically, first collect the sea sand, soak it in dilute nitric acid to remove microbial matter, and then wash it, soak the sample sand with a saturated solution of sodium chloride, inflate it for more than half an hour, then stop the aeration, let it stand for more than 24 hours, and then pour it out. The supernatant was filtered with a 0.45 micr...

Embodiment 3

[0033] An embodiment of the present invention provides a method for dyeing marine polluted microplastics, which includes the following steps:

[0034] Preparation of dye solution: mix methylene blue in ethanol solution at a concentration ratio of 1:1.5 to form a dye solution, in which the concentration of Coomassie brilliant blue is 0.1g / L, and the concentration of methylene blue is 0.15g / L, both in in ethanol solution;

[0035] Marine pollutant treatment: pre-select a certain amount of marine pollutants, and then select a mixture containing microplastics from marine pollutants. Specifically, sea sand and other marine pollutants are collected together, soaked in dilute nitric acid to remove microbial matter, and after washing, soak the sample in saturated sodium iodide solution, inflate for more than half an hour, then stop aerating, and let it stand for 24 hours. hours, then pour out the supernatant and filter it with a 0.45 micron organic filter.

[0036] Dyeing treatment:...

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Abstract

The invention relates to a marine pollutant micro-plastic dyeing method, comprising the steps of mixing Coomassie brilliant blue and methylene blue in an organic solvent to form a dye solution; selecting from marine pollutants, a mixture containing micro=plastic; dyeing the mixture with the dye solution, and keeping vibrating for dyeing for a predetermined period of time. The marine pollution micro-plastic dyeing method has the advantages that Coomassie brilliant blue and methylene blue are mixed in an organic solvent to obtain the dye solution, marine pollutants are dyed via the dye solution,dyeing time is short, dyeing acts fast, and dyeing is convenient and fast and is simple to perform. Compared with traditional high-cost Nile red, Coomassie brilliant blue and methylene blue are lowerin cost and are applicable to wide researches on marine pollutant micro-plastics.

Description

technical field [0001] The invention relates to the technical field of marine pollutant research, in particular to a method for dyeing marine polluted microplastics. Background technique [0002] Microplastics are currently the key research object of marine pollutants, and various places are stepping up research on the content of microplastics in the ocean. However, because microplastics are extremely stable, it is extremely difficult to react with various reagents, and most microplastics are transparent and difficult to observe. Therefore, the existing method is to find a suitable dye, and dyeing it in color is helpful for experimental research. At present, there are only reports in the literature that Nile Red can stain microplastics, but Nile Red is expensive and not suitable for extensive research. Therefore, it is necessary to develop a new low-cost method to dye microplastics. Contents of the invention [0003] In view of this, a low-cost, fast, efficient and conv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/205D06P1/00
CPCC08J3/2053C08J2300/30D06P1/00
Inventor 徐秋阳齐丹郑伟艾小琪
Owner HAINAN TROPICAL OCEAN UNIV
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