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Method for detecting id marks related to tumor in blood serum from patient of oesophagus cancer

A tumor-related, patient serum technology, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as inability to produce antibodies

Inactive Publication Date: 2006-12-06
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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Problems solved by technology

Since SEREX needs to establish an expression library, its analysis also has limitations in the genetic material of the tumor individual genome.
In addition, SEREX technology cannot generate antibodies against protein isoforms or different post-translational modified forms of proteins

Method used

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  • Method for detecting id marks related to tumor in blood serum from patient of oesophagus cancer
  • Method for detecting id marks related to tumor in blood serum from patient of oesophagus cancer
  • Method for detecting id marks related to tumor in blood serum from patient of oesophagus cancer

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Embodiment 1

[0019] see figure 1 and figure 2 A method for detecting tumor-associated markers in the serum of patients with esophageal cancer, the steps of which are as follows:

[0020] A. Collect peripheral blood samples from patients with esophageal cancer, centrifuge at 4°C within 2-3 hours after isolation, and separate the upper serum to obtain tumor serum;

[0021] B. Collect peripheral blood samples from healthy people, centrifuge at 4°C within 2-3 hours after isolation, and separate the upper serum to obtain control serum;

[0022] C, extract the total protein of cell lines cultured in vitro; the specific method of the present embodiment is: for the esophageal cancer cell line EC-0156 cultured in vitro, the culture solution in the culture dish is discarded, and pre-cooled 1 × phosphate buffered saline ( PBS), pH 7.4 (137mM NaCl, 2.7mM KCl, 10mM NaCl 2 HPO 4 , 2mM KH 2 PO 4 ), washed 3 times, added appropriate amount of protein lysate (50mM Tris-HCl, pH7.4, 150mM NaCl, 1% Tri...

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Abstract

The invention relates to a method to test tumor relative marking material from esophageal patient blood serum that uses blood serum as molecule probe. The Ku70 albumen would used as the new esophageal patient marking material. It is relative to early stage esophageal.

Description

technical field [0001] The invention relates to a method for detecting tumor-related markers in serum of patients with esophageal cancer. The method uses the serum of patients with esophageal cancer as antibodies to capture tumor antigens, and then enriches tumor antigen-antibody complexes. The enriched tumor antigen-antibody complexes are separated by SDS-PAGE electrophoresis and identified by mass spectrometry to obtain tumor markers. Background technique [0002] Esophageal cancer is one of the common malignant tumors in my country, and there is still a lack of effective early diagnosis methods. In the process of tumor development, the instability of the genome will cause many protein expression and degradation disorders. These abnormally changed proteins or small molecular peptides can be recognized by the body's immune system as "non-self" substances (self-antigens) and induce the body to produce anti-antigens. Humoral immune response to tumor autoantigens, with autoan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N33/574G01N33/561G01N33/531
Inventor 赵晓航何洪智吴旻
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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