Detection method for trace protein of Chinese medicinal injection

A protein detection and injection technology, which is used in biological testing, material testing products, and material analysis by observing the impact on chemical indicators to achieve high sensitivity, specificity, and strong anti-interference ability.

Inactive Publication Date: 2010-10-20
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Since traditional Chinese medicine injections have darker colors and various components, and proteins are a class of substances, it is difficult to improve the sensitivity and specificity of detection at the same time with the current single method

Method used

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  • Detection method for trace protein of Chinese medicinal injection
  • Detection method for trace protein of Chinese medicinal injection
  • Detection method for trace protein of Chinese medicinal injection

Examples

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Effect test

Embodiment Construction

[0022] Implementation Example 1

[0023] Establish PVDF membrane adsorption dyeing detection protein method, determine the detection limit of the present invention

[0024] Take 10 test tubes numbered 0-9, wash with PBS (KCl, 0.2g; KH 2 PO 4 , 0.2g; NaCl, 8.0g; Na 2 HPO 4 12H 2 O, 2.08g; add triple distilled water to 1000ml, pH 7.2) and BSA (bovine serum albumin) to prepare a BSA solution with a concentration of 9mg / ml in tube 0, and add 200 μl of PBS to tubes 1-9 , Take 100μl solution from tube No. 0 to tube No. 1 and mix evenly, then take 100μl solution from tube No. 1 to tube No. 2 and mix evenly, and so on to tube No. Group standard solution, tube No. 9 is the PBS blank control.

[0025] Mark the PVDF membrane and infiltrate it with methanol, spot 1 μl of the solution No. 0-9 on the PVDF membrane in turn, take pictures under the gel imaging system, and then stain with 0.25% Coomassie brilliant blue staining solution for 5 minutes, and decolorize it on a transfer deco...

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Abstract

The invention discloses a detection method for trace protein of a Chinese medicinal injection, which is used for detecting the protein impurities of the Chinese medicinal injection so as to control the quality of the Chinese medicinal injection. The detection method is characterized by comprising the following steps of: 1) applying a Chinese medicinal injection sample on a protein specific adsorption film; 2) washing the sample application film with organic solvent-containing solution to wash away the natural color of dots of the Chinese medicinal injection; and 3) subsequently performing coomassie brilliant blue staining on the sample application film. If the protein contained in the Chinese medicinal injection is no less than 12mu g/ml, stable and differentiable blue can be displayed on the film. The detection sensitivity of the method is higher than that of a sulfosalicylic acid precipitation method, and the detection method has high anti-interference capacity and is suitable for the detection of the trace protein of all the Chinese medicinal injections.

Description

Technical field [0001] The invention relates to the field of detection of impurities in traditional Chinese medicine injections, which is used for protein impurities in traditional Chinese medicine injections to control the quality of traditional Chinese medicine injections. technical background [0002] Traditional Chinese medicine injections were first produced in 1946 by scholars such as Qian Xinzhong. In the Chinese medical field, traditional Chinese medicine injections have been widely used clinically. The 2005 edition of "The Pharmacopoeia of the People's Republic of China" (abbreviated as "Chinese Pharmacopoeia") contains single and compound injections such as Erigeron breviscapus, Qingkailing, Zhichuanling and Shuanghuanglian. Traditional Chinese medicine injections have played an important role in promoting the development of traditional Chinese medicine formulations, expanding the scope of application of traditional Chinese medicine, and serving the health of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/78
Inventor 段为钢李奇峰卢国勋
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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