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Method for detecting trace protein of Chinese medicine injection

A protein detection and injection technology, applied in the direction of biological testing, material inspection products, material analysis by observing the influence of chemical indicators, etc.

Active Publication Date: 2011-02-16
石药银湖制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Since traditional Chinese medicine injections have darker colors and various components, and proteins are a class of substances, it is difficult to improve the sensitivity and specificity of detection at the same time with the current single method

Method used

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  • Method for detecting trace protein of Chinese medicine injection
  • Method for detecting trace protein of Chinese medicine injection
  • Method for detecting trace protein of Chinese medicine injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] Implementation example 1

[0025] Changing the detection method found that the detection sensitivity was improved

[0026] Take 13 1.5ml test tubes, tube numbers A0-A12, and use PBS (KCl, 0.2g; KH 2 PO 4 , 0.2g; NaCl, 8.0g; Na 2 HPO 4 12H 2 O, 2.08g; add triple distilled water to 1000ml, pH 7.2) and BSA (bovine serum albumin) to prepare 15ml of BSA solution with a concentration of 9mg / ml in tube 0, and add 1.0 ml PBS, take 0.5ml solution from No. 0 tube to No. 1 tube and mix well, then take 0.5ml solution from No. 1 tube to No. 2 tube and mix well, and so on to No. A12 tube to get the concentration gradient A set of standard protein solutions for 3 times, 1.0ml per tube. Tube A13 is a 1.0ml PBS blank control.

[0027] Cut the PVDF membrane into 9mm 2 The size of the membrane, soaked with methanol (about 10 seconds), put into A3-A9 and negative control tube A13, tightly cover the test tube, placed on the transfer decolorization shaker (30rpm, 20-25 ℃) overnight (...

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Abstract

The invention relates to a method for detecting trace protein of a Chinese medicine injection, which is used for detecting protein impurities in the Chinese medicine injection to control the quality of the Chinese medicine injection. The method is characterized by comprising the following steps of: 1) soaking a protein specific adsorption film in Chinese medicine injection solution to be detected; 2) washing the protein adsorption film by using solution containing an organic solvent to reduce color interference of the Chinese medicine injection; and 3) performing comassie brilliant blue colordevelopment on the protein adsorption film. If the protein contained in the Chinese medicine injection is not lower than 0.5 mu g / ml, the protein can display a stable and distinguishable blue color on the film. The method has high detection sensitivity, and simultaneously has strong interference resistance, so the method is suitable for detecting the trace protein of all Chinese medicine injections.

Description

Technical field [0001] The invention relates to the field of detection of impurities in traditional Chinese medicine injections, which is used for detecting protein impurities in Chinese medicine injections to control the quality of Chinese medicine injections. technical background [0002] Traditional Chinese medicine injections were first produced in the 1940s by scholars such as Qian Xinzhong. In China, traditional Chinese medicine injections have been widely used clinically. The 2010 edition of "The Pharmacopoeia of the People's Republic of China" (abbreviated as "Chinese Pharmacopoeia") contains single and compound injections such as Erigeron breviscapus, Qingkailing, Zhichuanling and Shuanghuanglian. Traditional Chinese medicine injections have played an important role in promoting the development of traditional Chinese medicine formulations, expanding the scope of application of traditional Chinese medicine, and serving the health of the general public. Safety, effe...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/78
Inventor 段为钢李奇峰柯瑾吕小满
Owner 石药银湖制药有限公司
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