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Full-spectrum super-resolution measuring method and label-free biomolecule measurement system

A super-resolution, measurement method technology, applied in color/spectral characteristic measurement, measurement device, optical device, etc., can solve the problems of complex instrument and high cost of consumables.

Active Publication Date: 2013-06-05
CAPITALBIO CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some studies have used interferometers to detect the reaction results of organic molecules in real time and without labels, but the harsh working conditions of white light interferometers limit the application of this instrument in the field of point-of-care testing (POCT).
In addition, there are other non-labeled biomolecular detection methods, such as surface plasmon resonance (SPR) method, electrical impedance method, etc., but these methods have encountered problems such as high cost of consumables, complicated instruments, etc., and cannot be used in real-time detection in the short term Domain (POCT) gets applied

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Embodiment Construction

[0039] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0040] Such as figure 1 , figure 2 As shown, the non-labeled biomolecular measurement system of the present invention includes a light source 1, a reflective optical microscopic imaging system 2, a silicon-based biochip 3, a two-dimensional electric translation stage 4, a spectrometer 5 and a computer 6, Reflective optical microscopic imaging system 2 comprises a condenser collimating mirror 21, a beam splitter 22, an objective lens 23 and an imaging lens 24, the input end of spectrometer 5 coincides with the focal point of imaging lens 24, and the output end of spectrometer 5 Connect to computer 6; during measurement, the two-dimensional electric translation stage 4 is placed directly below the objective lens 23, the silicon-based biochip 3 is fixed on the top of the two-dimensional electric translation stage 4, and the light emitted by the light s...

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PUM

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Abstract

The invention relates to a full-spectrum super-resolution measuring method and a label-free biomolecule measurement system. The method and the system are characterized in that membrane layers with different thicknesses ranging from 300nm to 4500nm are arranged on a chip and used for encoding, then sample application is carried out on the chip by means of probe protein to form a biological chip, the probe protein of the biological chip is fully combined with a to-be-tested sample, a to-be-tested object in the to-be-tested sample is arranged on the probe protein through specific adsorption, light beams with light intensity of I0 are vertically cast on the biological chip, when the light beams are vertically cast on the biological chip only covered with the membrane layers, different reflection spectrums are produced due to the different thicknesses of the membrane layers, thickness encoding of the biological chip is achieved, by means of an interference extremum method, the thicknesses t of the membrane layers of the biological chip are decoded according to wavelengths of extreme points in a light spectrum, when the light beams are cast on the to-be-tested object combined with the probe protein, thickness changes of the membrane layers in the range delta occur, intensity amplitude differences are obtained through calculation, and the thickness of the to-be-tested object is obtained. The method and the system can be widely used in thickness measurement of biomolecules.

Description

technical field [0001] The invention relates to a biomolecular thickness measurement method and a measurement system, in particular to a full-spectrum super-resolution measurement method and a non-labeled biomolecular measurement system. Background technique [0002] Due to its extremely high sensitivity and accuracy, the interference principle has been widely used in the precise measurement of film thickness. The earliest interferometer was jointly developed by American physicists Michelson and Morley in 1883, named Michelson interferometer. In the 1990s, with the maturity of microfabrication and digital imaging technology, white light interferometers began to appear. Unlike traditional Michelson interferometers which are only used to measure displacement, white light interferometers are mainly used to measure film thickness. The measurement arm and the reference arm of the white light interferometer are on the same side of the beam splitter, so the difference in length i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01B11/06G01N21/25
Inventor 黄国亮王同舟李强黎新赵松敏马丽罗贤波庞彪
Owner CAPITALBIO CORP
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