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Method for detecting trace of albumin in urine

An albumin and trace technology, applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve problems such as difficulty in popularization, pollution of the environment, and impact on the health of examiners, etc. To achieve the effect of simple technical operation, low cost and cost reduction

Inactive Publication Date: 2011-09-07
CENT SOUTH UNIV
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Problems solved by technology

Immunological methods require special monoclonal or polyclonal antibodies, and radioimmunoassays also need to use radioactive elements as tracers, which affects the health of examiners and seriously pollutes the environment; chromatography requires specific chromatographic columns and requires special There are greater difficulties in popularizing the instruments
At present, there is no easy-to-operate, low-cost, fast and accurate method for detecting microalbumin in urine

Method used

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  • Method for detecting trace of albumin in urine
  • Method for detecting trace of albumin in urine
  • Method for detecting trace of albumin in urine

Examples

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Embodiment 1

[0030] 1. Detection of microalbumin in urine samples

[0031] (a) According to the number of samples to be tested (each sample occupies a square of 8mm*8mm), cut a piece of PVDF film of suitable size, draw a ruler around the film with a pencil for sample addition, and mark it .

[0032] (b) Prepare albumin standard substances with serial concentrations of 12.5mg / L, 25mg / L, 50mg / L, 100mg / L, and 200mg / L.

[0033] (c) Equilibrate the urine sample to 37° C., centrifuge at 3000 g for 10 minutes, and take the supernatant as the sample to be tested.

[0034] (d) Adding samples: first add 2.5 μl of methanol to the center of a square, and then immediately add 2 μl of albumin standard or urine sample to be tested at the same position. If the added methanol has been blotted dry by the PVDF membrane but the standard or urine sample has not been successfully added, discard this grid and reload the sample in another grid. According to this method, both the standard substance and the urin...

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Abstract

The invention discloses a method for detecting a trace of albumin in urine, and the method comprises the following steps: adding methanol to a polyvinylidene fluoride or nitric acid cellulose membrane; after adding a series of albumin standard substances and a urine sample to be detected, staining by utilizing a Coomassie brilliant blue R250 staining agent and destaining by utilizing a destaining solution; after drying the membrane, comparing and analyzing the color shade of spots which are formed on the sample to be detected and the standard substances; carrying out the initial qualitative operation by virtue of the naked eyes; shooting or scanning the membrane; preparing a standard curve through software according to the concentrations of the standard substances and the average density of the spots; and obtaining the concentration of the sample to be detected through computing according to the average density of the spots of the sample to be detected, thereby realizing the quantitative analysis. The method disclosed by the invention has the advantages of small needed sample amount, simplicity and convenience in technical operation, reliable result and low cost, does not need special reagents and instruments and can be used for realizing the quantitative detection of a trace of albumin in the urine.

Description

technical field [0001] The invention relates to a method for detecting microalbumin in urine, in particular to a method for detecting albumin whose content is nanogram level. Background technique [0002] Urinary albumin excretion varies widely, from undetectable amounts to milligrams or even grams. Microalbuminuria (microalbunminuria, MAU) refers to the excretion rate of albumin in urine is higher than the normal level, but lower than the level that can be detected by conventional urine protein detection methods, that is, urinary albumin excretion is 20-200mg / L. Since the 1980s, the study of MAU has been paid attention to by people. A large number of clinical studies have shown that it is an important predictor of diabetic nephropathy and hypertensive nephropathy, an indicator of subclinical cardiovascular disease, and a sign of vascular endothelial cell dysfunction. Markers are sensitive indicators of preeclampsia and independent risk factors for venous thrombosis, etc....

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 徐克前向跃芸刘芳夏璐玲刘小兰
Owner CENT SOUTH UNIV
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