Oxytropis ehrig cinerea FEL4-OOc and separation method and application thereof
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A technology of Oxytropis spp., applied to Ehrigiospora Oxytropis FEL4-OOc, the preparation and application of this bacterium, which can solve the problems of lack of swainsonine sources and high price
Inactive Publication Date: 2009-09-30
NORTHWEST A & F UNIV
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Swainsonine has good prospects for developing new anti-tumor drugs, but the source of swainsonine is ve
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Embodiment 1
[0028] Example 1: Isolation of E. thorn bean FEL4-OOc
[0029] 1) Wash the dust on the surface of Acanthopanax japonica with deionized water from the whole plant grown on Nanhua Mountain, Haiyuan County, Ningxia, and separate the leaves, stems, flowers and species of the plant with sterilized tweezers, and then separate the leaves, The stems, flowers and seeds were wrapped with gauze respectively for surface disinfection.
[0030] 2) Use sterilized filter paper to absorb the water on the surface-sterilized plant tissue, and then cut the leaves, stems, flowers, seeds, etc. into 3mm 2 Then, each tissue block was inoculated on the surface of water agar medium, and cultured in an incubator at 18°C.
[0031] 3) When the mycelium grows from the edge of the tissue block, pick the mycelium at the edge of the colony and inoculate it on the surface of the enrichment medium, and place it in an incubator at 18°C for enrichment culture; The alkaloids were detected by thin-layer chromat...
Embodiment 2
[0032] Example 2: Identification of E. thorn bean FEL4-OOc
[0033] 1. Morphological characteristics
[0034] The colony of the FEL4-OOc strain was round, white at the initial stage of culture, and gradually changed to gray, gray-green, and black with the extension of the culture time, with a slight protrusion in the center ( figure 1 ). The mycelium has a transverse septum, but no mediastinum, and some old hyphae give birth to conidia, which have branches. Conidia and conidiophore with transverse septum, without mediastinum, the diaphragm of conidia is thick, the number of diaphragms varies from 2 to 5, spores are long rod-shaped, nearly cylindrical, and the size of conidia is 30- 90μm×5~10μm( figure 2 ).
[0035] 2) Characteristics of Cultivation
[0036]The strain grows very slowly on PDA, PCA, Czapek and other media, and its growth rates are 0.59, 0.46 and 0.42 mm / d, respectively. The optimum growth temperature is 18 °C, and the growth is inhibited when the temperatu...
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Abstract
The invention discloses an oxytropis ehrig fungus alternaria FEL4-OOc which is preserved in the China type culture collection center on October 8, 2008, and the preservation code is CCTCC NO: M 208143. Bacterial colony of bacterial strain is in a circular shape with regular edges, the bacterial colony is white during early culturing period and gradually changes to gray, celadon and black as the culturing time is prolonged, and the centre of the bacterial colony slightly protrudes. Mycelium has transverse septa but has no mediastinums, and is relatively dense. Conidiophores peduncles grow on Part of the aging hypha, and the conidiophores peduncles have braches. The conidiophores are less in amount and brown in color, and are singly distributed in a scattered mode. The conidiophores have the2-5 transverse septa with thicker transverse septa films but have no mediastinums, ; spores are in long rod shapes and column shapes with the size of 30 to 90 microns*5 to 10 microns. The bacterial strain is obtained from leaves, stems, flowers or seeds of yellowflower crazyweed herbs through manual separation and filtration, and has the function of synthesizing main toxin, i.e. spherosin of the yellowflower crazyweed herbs.
Description
technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a novel Embellisia oxytropis, in particular to a new Embellisia oxytropis capable of synthesizing Swainsonine (SW), the main toxin of locust weed FEL4-OOc also relates to the preparation and application of the bacterium. Background technique [0002] Locoweed is a general term for poisonous plants belonging to the genus Oxytropis and Astragalus, and it is also the most serious poisonous weed that harms grassland animal husbandry in the world. In my country, locust is widely distributed in the vast grasslands of northwest, north and southwest China, and the damage area reaches 11 million hectares. 2 , accounting for 2.8% of the country's grassland area. Acanthopanax japonica is one of the main poisonous plants growing on the grasslands of western my country. It mainly harms livestock such as horses, cattle, sheep, etc. It not only causes poisoning and death, but also causes...
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