Noninvasive detection kit for nasopharyngeal carcinoma susceptibility genes

Abstract

The invention provides a noninvasive detection kit for nasopharyngeal carcinoma susceptibility genes. The kit comprises a specific primer, a DNA sequencing primer, a PCR (Polymerase Chain Reaction) component, a PCR product purification component, a DNA sequencing reaction component and the like, wherein the specific primer is used for detecting the polymorphic site genotypes of four mononucleotides of the Rsall polymorphism on a CYP2E1 gene, the polymorphism of a G252 site on a TNF (Tumor Necrosis Factor)-alpha gene, the deletion (Present / Null) of a GSTM (Giant Solide Turner Of The Mediastinum) 1 gene and the deletion (Present / Null) of a GSTT (Gross Saponin From Tribulus Terrestris) 1 gene. According to the kit, the risk level of nasopharyngeal carcinoma of a subject is evaluated according to the genotypes of two mononucleotide polymorphic sites closely related to the nasopharyngeal carcinoma, and then, according to the gene detection results of each subject, the subjects are guided from the gene level to specifically prevent the nasopharyngeal carcinoma so as to reduce the morbidity risk rate of the nasopharyngeal carcinoma. The sampling method of the kit adopts oral mucosal cell sampling which is painless and non-invasive, and cross-infections are avoided. The sequencing detection result of the kit is accurate and reliable, and is easy to popularize because expensive import special instruments are not needed to be purchased.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, and specifically relates to a nasopharyngeal cancer susceptibility gene detection kit, which evaluates the risk level of nasopharyngeal cancer from the genetic level according to the detection results, and serves as a method for preventing and treating nasopharyngeal cancer. Cancer direction guide. Background technique [0002] Nasopharyngeal carcinoma refers to malignant tumors that occur on the roof and side walls of the nasopharyngeal cavity. Common clinical symptoms are nasal congestion, nasal discharge bleeding, headache, and tinnitus; late-stage invasion of the brain, tinnitus, deafness, headache, diplopia, and cervical lymphadenopathy may occur. In most countries in the world, the incidence of nasopharyngeal carcinoma is extremely low, but it is very high in some provinces and cities in southern China and some countries in southeastern Asia. Among th...

AI Technical Summary

Problems solved by technology

Studies have shown that the deletion of GSTT1 and GSTM1 genes leads to the deletion of their encoded isozymes, which may affect the body's detoxification function against the above-mentioned poisons and increase the host's sensitivity to the effects of carcinogens

Studies have shown that there are deletion polymorphisms in GSTM1 and GSTT1, and the deletion rate varies in different regions. The deletion rate of GSTM1 in Chinese is high, ranging from 35% to 63%, especially the deletion rate of GSTT1 is as high as 58%. This part of the population is equivalent to an increase of Concentrations of environmental toxins and carcinogens that increase an individual's risk of developing a range of types of cancer

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