Tissue culture method for fast propagation of primula denticulata ssp.sino-denticulata

A technology of tissue culture rapid propagation and ball flowering, which is applied in the field of plant tissue culture, can solve the problems that the method of division propagation cannot meet the production needs, and there is no relevant research and report on tissue culture, so as to shorten the seedling cycle and have strong consistency , the effect of easy seedling growth

Inactive Publication Date: 2011-05-11
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to seed propagation, Primula belongs to asexual propagation, available methods are: branch (leaf cluster) propagation, tissue culture propagation, a small number of perennial species can be propagated by cuttings, but the method of branch propagation is far from meeting production needs
[0003] Tissue culture is an important way to rapidly propagate good plant varieties. There have been many reports on tissue culture of Primula, but there is no relevant report on tissue culture of Primula denticulata ssp.sino-denticulata. Therefore, it is necessary to study the tissue culture of Primula denticulata ssp.sino-denticulata and form a set of specialized rapid propagation technology system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Using the leaves of Primula denticulata ssp.sino-denticulata as explants to explore the optimal medium for adventitious bud induction: the leaves of well-growing seedlings were used as explants to explore the optimal medium formula for adventitious bud induction. Take the young leaves of Primuladenticulata ssp.sino-denticulata and wash them with tap water for 5-6 hours, drop a drop of detergent in the water and shake for 15 minutes, gently brush off surface impurities with a soft brush, and rinse them with tap water repeatedly; Surface disinfection: disinfect with 70% alcohol for 15-20 seconds, rinse with sterile water for 4-5 times, disinfect with 0.1% mercury chloride solution for 4-5 minutes, and rinse with sterile water for 4-5 times. The sterilized leaves were then cut into 1 cm 2 Small pieces (with part of the veins) were inoculated on the induction medium of clustered buds, 5-6 pieces were inoculated in each bottle, and 10 bottles were connected to each medium, a...

Embodiment 2

[0027] The axillary buds of Primula denticulata ssp.sino-denticulata in northern Yunnan were used as explants for primary culture and subculture: select axillary buds from robust plants, rinse them with tap water for 5-6 hours, and disinfect the same process as the leaves , inoculated on MS+6-BA 2.0mg / l+NAA0.1mg / l cluster bud induction medium, inoculated 2 to 3 buds per bottle; the light conditions were natural scattered light 3000Lux plus artificial auxiliary light source 1800±200Lux, light Time 14h.

[0028] When the axillary buds are inoculated on the differentiation medium, new leaves are continuously pulled out. From 10 days on, the petioles of the new leaves are thick and the base turns red, and light green callus is gradually formed. After 2 weeks, adventitious buds begin to differentiate; 40 days after inoculation , A large number of adventitious buds are differentiated on the newly drawn petioles and leaves of the axillary buds.

[0029]Statistical results show that ...

Embodiment 3

[0031] Rooting culture of Primula denticulata ssp.sino-denticulata tissue culture seedlings: when the adventitious buds grew 2-3 leaves on the differentiation medium, they were excised from the callus and transferred to Rooting medium, formula: MS, MS+NAA (0.1mg / l, 0.2mg / l, 0.5mg / l), pH 5.8; light conditions: natural scattered light 3000Lux plus artificial auxiliary light source 2500Lux, light time 14h .

[0032] From the perspective of the time required for rooting, the time required for rooting in medium MS and MS+0.2mg / l NAA is the shortest, 8-10 days, but in MS+0.2mg / l NAA, the rooting amount of tissue culture seedlings And growth is obviously better than MS basic medium, the average height of tissue culture seedlings can reach 4.8cm, and the rooting rate is over 95%.

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Abstract

The invention provides a method for tissue culturing and fast propagating Primula denticulata ssp.sino-denticulata, comprising explant selection, surface disinfection, adventitious bud induction, subculture, rooting culture and test-tube seedling transplanting. The method is characterized in that one axillary bud can induce about 40-50 sprouts, the multiplication coefficient is about 3- 4 after 30- 40 days subculture, the rooting rate reaches above 95%, and the replanting survival rate is nearly 100%. The invention realizes preservation for single Primula denticulata ssp.sino-denticulata, meanwhile greatly increases seedling capacity for Primula denticulata ssp.sino-denticulata i, reduces seedling time and cost, and provides technique support for Primula denticulata ssp.sino-denticulata application in large areas.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a tissue culture rapid propagation method of Primula denticulata ssp. sino-denticulata. Background technique [0002] Primula denticulata (Primula denticulata ssp. sino-denticulata) is a plant of the genus Primulaceae Primulaceae, and it is a very beautiful garden ornamental flower. Primula denticulata ssp.sino-denticulata is a perennial herbaceous flower. The whole plant is in the shape of a rosette, with well-developed rhizomes and 5 or more leaf clumps growing upwards. It is mainly propagated by seeding, usually in spring Afterwards, after vegetative growth, it begins to flower at the end of February of the following year, with slow growth and low reproduction coefficient. Except seeding propagation, Primula belongs to asexual propagation, available methods are: branch (leaf cluster) propagation, tissue culture propagation, a few perennial species can be propagated by cuttings, but the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G7/06A01G1/00
Inventor 张启翔李翠娟潘会堂梁树乐程堂仁孙明
Owner BEIJING FORESTRY UNIVERSITY
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