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An improved mesenchymal stem cell protection solution and its application

A technology of mesenchymal stem cells and protective solution, applied in the field of improved mesenchymal stem cell protective solution, can solve the problems of short time and limited application scope of mesenchymal stem cells.

Active Publication Date: 2014-10-22
青岛奥克生物开发有限公司
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Problems solved by technology

[0007] Another disadvantage of the prior art is that it maintains the viability of mesenchymal stem cells for a short period of time. Mesenchymal stem cells can maintain more than 90% of their viability in the cell preservation solution of the prior art for only 24 hours, and with time Prolonged, the cell viability decreases, which means that the cell viability will drop below 90% after 24 hours. However, stem cell therapy and clinical trials have been carried out in most cities in China and many foreign countries. Using existing means of transportation, 24 hours The viability maintenance time of mesenchymal stem cells obviously limits the application range of mesenchymal stem cells

Method used

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  • An improved mesenchymal stem cell protection solution and its application
  • An improved mesenchymal stem cell protection solution and its application
  • An improved mesenchymal stem cell protection solution and its application

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Embodiment Construction

[0027] 1. Isolation, culture and phenotypic identification of umbilical cord mesenchymal stem cells

[0028] (1) Soak the umbilical cord in sterile PBS buffer (Solarbio) containing 100 IU / L penicillin and 100 mg / L streptomycin for 10-15 minutes.

[0029] (2) Fully wash the umbilical cord with the above-mentioned sterile PBS buffer solution containing penicillin and streptomycin, and cut the umbilical cord tissue pieces into 1mm pieces as much as possible. 3 small pieces.

[0030] (3) The shredded tissue was added to DMEM medium (Gibco, USA) for washing, centrifuged at 2000 rpm for 10 minutes, and the supernatant was discarded.

[0031] (4) Add trypsin (Sigma Company, USA) into DMEM medium to prepare a digestion solution with a final concentration of 0.25% (volume ratio), suspend the tissue pieces with the digestion solution, and place them in a 37°C incubator for 30 minutes for digestion , centrifuge, and discard the supernatant.

[0032] (5) Add DMEM medium to suspend the ...

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Abstract

The invention relates to an improved mesenchyme stem cell protection solution as well as application and a preparation method thereof. The protection solution can effectively prolong the activity remaining time of mesenchyme stem cells, reduces preparation cost, and has the advantages of wide raw material source, simple preparation, safe and reliable direct clinical application; and after the mesenchyme stem cells are preserved for 48 hours in the protection solution, the cell activity is still above 90 percent, the cell morphology is normal, and the multiplication capacity and mesenchyme stem cell phenotype characteristics are not influenced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an improved mesenchymal stem cell protection solution, its application and its preparation method. technical background [0002] Mesenchymal stem cells (MSCs) are a kind of stem cells derived from mesoderm with multi-lineage differentiation ability, which widely exist in various tissues of the body, can be cultured and expanded in vitro, and can differentiate into Cardiomyocytes, nerve cells and fat cells, etc. With the in-depth research on the biological characteristics and functions of mesenchymal stem cells by scholars at home and abroad, mesenchymal stem cells can be isolated from various tissues. Mesenchymal stem cells can support hematopoiesis and regulate immunity, and have broad clinical application prospects in hematopoietic reconstruction, tissue repair, and immunotherapy. Mesenchymal stem cells do not express MHC class II molecules and co-stimulatory factors ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A01N1/02
Inventor 黄玉香高宏王丽胡建霞张学峰张美荣
Owner 青岛奥克生物开发有限公司
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