Tissue treatment device for protein purification

Abstract

The invention relates to the field of protein purification and particularly relates to a tissue treatment device for protein purification. The technical problem to be solved by the invention is to provide the tissue treatment device for protein purification. The tissue treatment device for protein purification comprises a machine body bottom plate, an isolation sampling mechanism, an internal tissue sampling mechanism, a tissue cell breaking mechanism, an external sealing communication mechanism, an operation control screen and the like, wherein the top left side of the body bottom plate is welded to the top deck. The tissue treatment device is advantaged in that the sterile sample input is realized, sealing, isolating and sampling after disinfection of the sample are completed, the deviceextends into original sample tissues to extract internal fresh tissues, cell characteristics are unchanged, protein is invariant, interference of original impurities and bacteria on an outer surfaceof a sample is prevented, fine grinding after mincing is achieved, the device is always in a sealed and isolated state before and after treatment, and sterile cell mixed liquid is obtained.

Description

technical field [0001] The invention relates to the field of protein purification, in particular to a tissue processing device for protein purification. Background technique [0002] The separation and purification of proteins is widely used in biochemical research applications and is an important operational technique. A typical eukaryotic cell can contain thousands of different proteins, some abundant and some with only a few copies. In order to study a protein, it must first be purified from other proteins and non-protein molecules. [0003] In the prior art, most protein purification is to directly collect cells and tissues for subsequent processing, but most of the collected biological samples are samples stored in the laboratory. A small piece of sample tissue is separated from the surface. Although the sample may be stored in a sterile laboratory, the moment the sample comes into contact with the air, the bacterial spores in the air will stick to the surface of the ...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the fact that most of the collected biological samples are samples stored in the laboratory, the present invention separates a small piece of sample tissue from the outer surface of the sample tissue through tools such as tweezers and scissors during manual sample collection, although the sample may be stored in Sterile laboratory, but the moment the sample is in contact with the air, the bacterial spores in the air will stick to the surface of the tissue, and at the same time, the sample is manually taken and processed, completely exposed to the air environment, and physical single cells are often used in the tissue cell processing stage. The lysis method, which in turn led to a large number of bacteria and fungi adhering to some samples obtained from the tissue sample, and the outer surface of the sample tissue was in contact with the air for a long time, even under cold storage conditions, it still caused slight oxidation and protein denaturation, resulting in the final The purified protein is not the target protein, and some characteristic functions of the protein are lost. At the same time, a single physical cell lysis method cannot lyse specific organelles inside the cell, and furthermore, some cellular proteins cannot be extracted. The technical problem to be solved by the present invention is to provide a Tissue processing device for protein purification

Images