Tissue treatment device for protein purification

A tissue processing and protein purification technology, applied in the field of protein purification, which can solve problems such as protein denaturation, loss of protein properties and functions, and oxidation.

Pending Publication Date: 2020-10-23
李庆华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the fact that most of the collected biological samples are samples stored in the laboratory, the present invention separates a small piece of sample tissue from the outer surface of the sample tissue through tools such as tweezers and scissors during manual sample collection, although the sample may be stored in Sterile laboratory, but the moment the sample is in contact with the air, the bacterial spores in the air will stick to the surface of the tissue, and at the same time, the sample is manually taken and processed, completely exposed to the air environment, and physical single cells are often used in the tissue cell processing stage. The lysis method, which in tur

Method used

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  • Tissue treatment device for protein purification
  • Tissue treatment device for protein purification
  • Tissue treatment device for protein purification

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Embodiment

[0031] A tissue processing device for protein purification, such as Figure 1-7 As shown, it includes a body bottom plate 1, a top deck 2, a first connecting deck 3, a second connecting deck 4, a third connecting deck 5, an isolation sampling mechanism 6, an internal tissue sampling mechanism 7, and a tissue cell crushing mechanism 8 , externally connected to the sealed communication mechanism 9 and the operation control panel 10; the top left side of the body bottom plate 1 is welded to the top deck 2; the top right side of the body bottom plate 1 is welded to the first connecting deck 3; the top left side of the body bottom plate 1 is isolated from the The sampling mechanism 6 is connected, and the left middle part of the isolation sampling mechanism 6 is connected with the top deck 2; the top right side of the bottom plate 1 of the body is connected with the tissue cell crushing mechanism 8, and the right lower part of the tissue cell crushing mechanism 8 is connected with t...

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Abstract

The invention relates to the field of protein purification and particularly relates to a tissue treatment device for protein purification. The technical problem to be solved by the invention is to provide the tissue treatment device for protein purification. The tissue treatment device for protein purification comprises a machine body bottom plate, an isolation sampling mechanism, an internal tissue sampling mechanism, a tissue cell breaking mechanism, an external sealing communication mechanism, an operation control screen and the like, wherein the top left side of the body bottom plate is welded to the top deck. The tissue treatment device is advantaged in that the sterile sample input is realized, sealing, isolating and sampling after disinfection of the sample are completed, the deviceextends into original sample tissues to extract internal fresh tissues, cell characteristics are unchanged, protein is invariant, interference of original impurities and bacteria on an outer surfaceof a sample is prevented, fine grinding after mincing is achieved, the device is always in a sealed and isolated state before and after treatment, and sterile cell mixed liquid is obtained.

Description

technical field [0001] The invention relates to the field of protein purification, in particular to a tissue processing device for protein purification. Background technique [0002] The separation and purification of proteins is widely used in biochemical research applications and is an important operational technique. A typical eukaryotic cell can contain thousands of different proteins, some abundant and some with only a few copies. In order to study a protein, it must first be purified from other proteins and non-protein molecules. [0003] In the prior art, most protein purification is to directly collect cells and tissues for subsequent processing, but most of the collected biological samples are samples stored in the laboratory. A small piece of sample tissue is separated from the surface. Although the sample may be stored in a sterile laboratory, the moment the sample comes into contact with the air, the bacterial spores in the air will stick to the surface of the ...

Claims

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Application Information

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IPC IPC(8): G01N1/04G01N1/28
CPCG01N1/04G01N1/286G01N2001/2866
Inventor 李庆华
Owner 李庆华
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