A double umbilical snail SNP molecular marker and its application in traceability
A technology of molecular markers and double-umbilical snails, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of small differences in allele distribution frequency, large heterozygosity, and high variability, and achieve The effect of rich polymorphism and small difference in frequency distribution
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Embodiment 1
[0030] Example 1 Search of SNP molecular markers
[0031] (1) Construction of DNA pool (pool)
[0032] Two umbilical snails in Shenzhen, Dongguan and Hong Kong were randomly collected respectively, and after the genomic DNA was extracted, the DNA of 6 individuals was aspirated and put into the same centrifuge tube, and mixed well for use.
[0033] (2) Primer design
[0034] Using the DNA sequence of the LOC106079843 gene of the umbilical snail (Genbank: NW_013487738.1) as a template, primers were designed to isolate the DNA fragment of the LOC106079843 gene of the umbilical snail. The primers are as follows:
[0035] Upstream primer: 5´-AAGAGTTGTGAGAGTGGGCC-3´ (as shown in SEQ ID NO.2)
[0036] Downstream primer: 5´-TCGGCCGAAAGTCATTATATGG-3´ (as shown in SEQ ID NO.3)
[0037] The total volume of the PCR reaction was 50 μL, including about 100 ng of A. umbilicus genomic DNA, 45 μL of 1.1×PCR mix, and 2 μL of 10 μmol / L upstream and downstream primers. The PCR amplification p...
Embodiment 2
[0044] Example 2 Distribution of alleles
[0045] (1) Design of test groups
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